TY - JOUR
T1 - Multiphoton microscopy
T2 - An introduction to gastroenterologists
AU - Cho, Hye Jin
AU - Chun, Hoon Jai
AU - Kim, Eun Sun
AU - Cho, Bong Rae
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - Multiphoton microscopy, relying on the simultaneous absorption of two or more photons by a fuorophore, has come to occupy a prominent place in modern biomedical research with its ability to allow real-time observation of a single cell and molecules in intact tissues. Multiphoton microscopy exhibits nonlinear optical contrast properties, which can make it possible to provide an exceptionally large depth penetration with less phototoxicity. This system becomes more and more an inspiring tool for a non-invasive imaging system to realize "optical biopsy" and to examine the functions of living cells. In this review, we briefly present the physical principles and properties of multiphoton microscopy as well as the current applications in biological fields. In addition, we address what we see as the future potential of multiphoton microscopy for gastroenterologic research.
AB - Multiphoton microscopy, relying on the simultaneous absorption of two or more photons by a fuorophore, has come to occupy a prominent place in modern biomedical research with its ability to allow real-time observation of a single cell and molecules in intact tissues. Multiphoton microscopy exhibits nonlinear optical contrast properties, which can make it possible to provide an exceptionally large depth penetration with less phototoxicity. This system becomes more and more an inspiring tool for a non-invasive imaging system to realize "optical biopsy" and to examine the functions of living cells. In this review, we briefly present the physical principles and properties of multiphoton microscopy as well as the current applications in biological fields. In addition, we address what we see as the future potential of multiphoton microscopy for gastroenterologic research.
KW - Gastrointestinal disease
KW - Multiphoton microscopy
KW - Optical biopsy
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U2 - 10.3748/wjg.v17.i40.4456
DO - 10.3748/wjg.v17.i40.4456
M3 - Editorial
C2 - 22110275
AN - SCOPUS:81755181912
VL - 17
SP - 4456
EP - 4460
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 40
ER -