Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CDld

Ya Hui Chiu, Se Ho Park, Kamel Benlagha, Claire Forestier, Jayanthi Jayawardena-Wolf, Paul B. Savage, Luc Teyton, Albert Bendelac

Research output: Contribution to journalArticlepeer-review

164 Citations (Scopus)

Abstract

For members of the CD I family of β2-microglobulin-associated lipid-presenting molecules, tyrosine-based motifs in the cytoplasmic tail and invariant chain (Ii) govern glycoprotein trafficking through endosomal compartments. Little is known about the intracellular pathways of CD I trafficking and antigen presentation. However, in vitro studies with cells transfected with mutant CD I that had a truncated cytoplasmic tail have suggested a role for these tyrosine motifs in some, but not all, antigenic systems. By introducing a deletion of the tyrosine motif into the germ line, and through homologous recombination in embryonic stem cells, we now describe knock-in mice with the CD I d cytoplasmic tail deleted. Despite adequate surface CD I d expression and the presence of Ii, these mutant mice showed multiple and selective abnormalities in intracellular trafficking, antigen presentation and T cell development, demonstrating the critical functions of the CD I d cytoplasmic tail motif in vivo.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalNature Immunology
Volume3
Issue number1
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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