Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CDld

Ya Hui Chiu, Se-Ho Park, Kamel Benlagha, Claire Forestier, Jayanthi Jayawardena-Wolf, Paul B. Savage, Luc Teyton, Albert Bendelac

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

For members of the CD I family of β 2-microglobulin-associated lipid-presenting molecules, tyrosine-based motifs in the cytoplasmic tail and invariant chain (Ii) govern glycoprotein trafficking through endosomal compartments. Little is known about the intracellular pathways of CD I trafficking and antigen presentation. However, in vitro studies with cells transfected with mutant CD I that had a truncated cytoplasmic tail have suggested a role for these tyrosine motifs in some, but not all, antigenic systems. By introducing a deletion of the tyrosine motif into the germ line, and through homologous recombination in embryonic stem cells, we now describe knock-in mice with the CD I d cytoplasmic tail deleted. Despite adequate surface CD I d expression and the presence of Ii, these mutant mice showed multiple and selective abnormalities in intracellular trafficking, antigen presentation and T cell development, demonstrating the critical functions of the CD I d cytoplasmic tail motif in vivo.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalNature Immunology
Volume3
Issue number1
DOIs
Publication statusPublished - 2002 Jan 26

Fingerprint

CD-I
Antigen Presentation
Tail
T-Lymphocytes
Tyrosine
CD Antigens
Multiple Abnormalities
Homologous Recombination
Embryonic Stem Cells
Germ Cells
Glycoproteins
Lipids

ASJC Scopus subject areas

  • Immunology

Cite this

Chiu, Y. H., Park, S-H., Benlagha, K., Forestier, C., Jayawardena-Wolf, J., Savage, P. B., ... Bendelac, A. (2002). Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CDld. Nature Immunology, 3(1), 55-60. https://doi.org/10.1038/ni740

Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CDld. / Chiu, Ya Hui; Park, Se-Ho; Benlagha, Kamel; Forestier, Claire; Jayawardena-Wolf, Jayanthi; Savage, Paul B.; Teyton, Luc; Bendelac, Albert.

In: Nature Immunology, Vol. 3, No. 1, 26.01.2002, p. 55-60.

Research output: Contribution to journalArticle

Chiu, YH, Park, S-H, Benlagha, K, Forestier, C, Jayawardena-Wolf, J, Savage, PB, Teyton, L & Bendelac, A 2002, 'Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CDld', Nature Immunology, vol. 3, no. 1, pp. 55-60. https://doi.org/10.1038/ni740
Chiu, Ya Hui ; Park, Se-Ho ; Benlagha, Kamel ; Forestier, Claire ; Jayawardena-Wolf, Jayanthi ; Savage, Paul B. ; Teyton, Luc ; Bendelac, Albert. / Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CDld. In: Nature Immunology. 2002 ; Vol. 3, No. 1. pp. 55-60.
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