Multiple GO-SELEX for efficient screening of flexible aptamers

Van Thuan Nguyen, Young Seop Kwon, Jae Hoon Kim, Man Bock Gu

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We describe a simple, high-speed, high-throughput aptamer screening for a group of small molecules using graphene oxide (simple Multi-GO-SELEX) without immobilizing targets. The affinities of ten different ssDNA aptamers successfully obtained for three pesticides were in the range of 10–100 nM. Besides a specific aptamer for each target, we found a couple of flexible multi-target aptamers, which can bind with 2 or 3 different molecules. These flexible aptamers developed for binding with a mixture of targets are not only significant for the rapid screening of a group of small molecules but also offer great promise for aptamer-based biosensor applications.

Original languageEnglish
Pages (from-to)10513-10516
Number of pages4
JournalChemical Communications
Volume50
Issue number72
DOIs
Publication statusPublished - 2014 Aug 12

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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