Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Xinglu Huang, Magdalena Swierczewska, Ki Young Choi, Lei Zhu, Ashwinkumar Bhirde, Jinwoo Park, Kwangmeyung Kim, Jin Xie, Gang Niu, Kang Choon Lee, Seulki Lee, Xiaoyuan Chen

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

Original languageEnglish
Pages (from-to)1625-1630
Number of pages6
JournalAngewandte Chemie - International Edition
Volume51
Issue number7
DOIs
Publication statusPublished - 2012 Feb 13
Externally publishedYes

Keywords

  • caspases
  • fluorescence
  • imaging agents
  • nanoparticles
  • proteolysis

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher'. Together they form a unique fingerprint.

  • Cite this

    Huang, X., Swierczewska, M., Choi, K. Y., Zhu, L., Bhirde, A., Park, J., Kim, K., Xie, J., Niu, G., Lee, K. C., Lee, S., & Chen, X. (2012). Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher. Angewandte Chemie - International Edition, 51(7), 1625-1630. https://doi.org/10.1002/anie.201107795