Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Xinglu Huang, Magdalena Swierczewska, Ki Young Choi, Lei Zhu, Ashwinkumar Bhirde, Jinwoo Park, Kwang Meyung Kim, Jin Xie, Gang Niu, Kang Choon Lee, Seulki Lee, Xiaoyuan Chen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

Original languageEnglish
Pages (from-to)1625-1630
Number of pages6
JournalAngewandte Chemie - International Edition
Volume51
Issue number7
DOIs
Publication statusPublished - 2012 Feb 13
Externally publishedYes

Fingerprint

Coloring Agents
Dyes
Proteolysis
Imaging techniques
Silicon Dioxide
Fluorescence
Silica
Nanoparticles
Infrared radiation
Substrates

Keywords

  • caspases
  • fluorescence
  • imaging agents
  • nanoparticles
  • proteolysis

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher. / Huang, Xinglu; Swierczewska, Magdalena; Choi, Ki Young; Zhu, Lei; Bhirde, Ashwinkumar; Park, Jinwoo; Kim, Kwang Meyung; Xie, Jin; Niu, Gang; Lee, Kang Choon; Lee, Seulki; Chen, Xiaoyuan.

In: Angewandte Chemie - International Edition, Vol. 51, No. 7, 13.02.2012, p. 1625-1630.

Research output: Contribution to journalArticle

Huang, X, Swierczewska, M, Choi, KY, Zhu, L, Bhirde, A, Park, J, Kim, KM, Xie, J, Niu, G, Lee, KC, Lee, S & Chen, X 2012, 'Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher', Angewandte Chemie - International Edition, vol. 51, no. 7, pp. 1625-1630. https://doi.org/10.1002/anie.201107795
Huang, Xinglu ; Swierczewska, Magdalena ; Choi, Ki Young ; Zhu, Lei ; Bhirde, Ashwinkumar ; Park, Jinwoo ; Kim, Kwang Meyung ; Xie, Jin ; Niu, Gang ; Lee, Kang Choon ; Lee, Seulki ; Chen, Xiaoyuan. / Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher. In: Angewandte Chemie - International Edition. 2012 ; Vol. 51, No. 7. pp. 1625-1630.
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