Multiplex polymerase chain reaction test for the diagnosis of acute viral hepatitis A.

Nae Yun Heo, Young Suk Lim, Jihyun An, Sun-Young Ko, Heung Bum Oh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The early diagnosis of acute hepatitis A (AHA) is hindered because serum IgM against hepatitis A virus (HAV) can yield false-negative results during the window period. This study evaluated the diagnostic accuracy of a polymerase chain reaction (PCR) kit for HAV RNA for the diagnosis of AHA. Samples were collected from 136 patients with acute severe hepatitis at their admission to Asan Medical Center between June 2010 and July 2010. Samples were analyzed for serum IgM anti-HAV using an immunoassay test and for qualitative HAV RNA using the Magicplex HepaTrio PCR test kit. The diagnostic accuracies of these methods were tested on the basis of clinical and laboratory diagnoses of AHA. The concordance rate and kappa value between IgM anti-HAV and HAV RNA PCR were 88.2% and 0.707, respectively. For the diagnosis of AHA, the sensitivity and specificity of IgM anti-HAV were 90.7% and 100%, respectively, when an "equivocal" result was regarded as positive; and 79.1% and 100%, respectively, when an "equivocal" result was regarded as negative. The sensitivity and specificity of HAV RNA PCR were 81.4% and 100%, respectively. All four patients with negative IgM anti-HAV and positive HAV RNA PCR results and all four patients with equivocal IgM anti-HAV RNA and positive HAV RNA PCR results were eventually diagnosed with AHA. The qualitative HAV RNA PCR test has an equivalent diagnostic accuracy for AHA compared to IgM anti-HAV and may be more sensitive during the window period.

Original languageEnglish
Pages (from-to)397-403
Number of pages7
JournalClinical and molecular hepatology
Volume18
Issue number4
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes

Fingerprint

Hepatitis A virus
Hepatitis A
Multiplex Polymerase Chain Reaction
DNA-Directed RNA Polymerases
Polymerase Chain Reaction
RNA
Sensitivity and Specificity
Clinical Laboratory Techniques
Serum
Immunoassay
Hepatitis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Multiplex polymerase chain reaction test for the diagnosis of acute viral hepatitis A. / Heo, Nae Yun; Lim, Young Suk; An, Jihyun; Ko, Sun-Young; Oh, Heung Bum.

In: Clinical and molecular hepatology, Vol. 18, No. 4, 01.12.2012, p. 397-403.

Research output: Contribution to journalArticle

Heo, Nae Yun ; Lim, Young Suk ; An, Jihyun ; Ko, Sun-Young ; Oh, Heung Bum. / Multiplex polymerase chain reaction test for the diagnosis of acute viral hepatitis A. In: Clinical and molecular hepatology. 2012 ; Vol. 18, No. 4. pp. 397-403.
@article{6140bcd8bd424f1abecf9c7584d72681,
title = "Multiplex polymerase chain reaction test for the diagnosis of acute viral hepatitis A.",
abstract = "The early diagnosis of acute hepatitis A (AHA) is hindered because serum IgM against hepatitis A virus (HAV) can yield false-negative results during the window period. This study evaluated the diagnostic accuracy of a polymerase chain reaction (PCR) kit for HAV RNA for the diagnosis of AHA. Samples were collected from 136 patients with acute severe hepatitis at their admission to Asan Medical Center between June 2010 and July 2010. Samples were analyzed for serum IgM anti-HAV using an immunoassay test and for qualitative HAV RNA using the Magicplex HepaTrio PCR test kit. The diagnostic accuracies of these methods were tested on the basis of clinical and laboratory diagnoses of AHA. The concordance rate and kappa value between IgM anti-HAV and HAV RNA PCR were 88.2{\%} and 0.707, respectively. For the diagnosis of AHA, the sensitivity and specificity of IgM anti-HAV were 90.7{\%} and 100{\%}, respectively, when an {"}equivocal{"} result was regarded as positive; and 79.1{\%} and 100{\%}, respectively, when an {"}equivocal{"} result was regarded as negative. The sensitivity and specificity of HAV RNA PCR were 81.4{\%} and 100{\%}, respectively. All four patients with negative IgM anti-HAV and positive HAV RNA PCR results and all four patients with equivocal IgM anti-HAV RNA and positive HAV RNA PCR results were eventually diagnosed with AHA. The qualitative HAV RNA PCR test has an equivalent diagnostic accuracy for AHA compared to IgM anti-HAV and may be more sensitive during the window period.",
author = "Heo, {Nae Yun} and Lim, {Young Suk} and Jihyun An and Sun-Young Ko and Oh, {Heung Bum}",
year = "2012",
month = "12",
day = "1",
doi = "10.3350/cmh.2012.18.4.397",
language = "English",
volume = "18",
pages = "397--403",
journal = "Clinical and molecular hepatology",
issn = "2287-2728",
publisher = "Korean Association for the Study of the Liver",
number = "4",

}

TY - JOUR

T1 - Multiplex polymerase chain reaction test for the diagnosis of acute viral hepatitis A.

AU - Heo, Nae Yun

AU - Lim, Young Suk

AU - An, Jihyun

AU - Ko, Sun-Young

AU - Oh, Heung Bum

PY - 2012/12/1

Y1 - 2012/12/1

N2 - The early diagnosis of acute hepatitis A (AHA) is hindered because serum IgM against hepatitis A virus (HAV) can yield false-negative results during the window period. This study evaluated the diagnostic accuracy of a polymerase chain reaction (PCR) kit for HAV RNA for the diagnosis of AHA. Samples were collected from 136 patients with acute severe hepatitis at their admission to Asan Medical Center between June 2010 and July 2010. Samples were analyzed for serum IgM anti-HAV using an immunoassay test and for qualitative HAV RNA using the Magicplex HepaTrio PCR test kit. The diagnostic accuracies of these methods were tested on the basis of clinical and laboratory diagnoses of AHA. The concordance rate and kappa value between IgM anti-HAV and HAV RNA PCR were 88.2% and 0.707, respectively. For the diagnosis of AHA, the sensitivity and specificity of IgM anti-HAV were 90.7% and 100%, respectively, when an "equivocal" result was regarded as positive; and 79.1% and 100%, respectively, when an "equivocal" result was regarded as negative. The sensitivity and specificity of HAV RNA PCR were 81.4% and 100%, respectively. All four patients with negative IgM anti-HAV and positive HAV RNA PCR results and all four patients with equivocal IgM anti-HAV RNA and positive HAV RNA PCR results were eventually diagnosed with AHA. The qualitative HAV RNA PCR test has an equivalent diagnostic accuracy for AHA compared to IgM anti-HAV and may be more sensitive during the window period.

AB - The early diagnosis of acute hepatitis A (AHA) is hindered because serum IgM against hepatitis A virus (HAV) can yield false-negative results during the window period. This study evaluated the diagnostic accuracy of a polymerase chain reaction (PCR) kit for HAV RNA for the diagnosis of AHA. Samples were collected from 136 patients with acute severe hepatitis at their admission to Asan Medical Center between June 2010 and July 2010. Samples were analyzed for serum IgM anti-HAV using an immunoassay test and for qualitative HAV RNA using the Magicplex HepaTrio PCR test kit. The diagnostic accuracies of these methods were tested on the basis of clinical and laboratory diagnoses of AHA. The concordance rate and kappa value between IgM anti-HAV and HAV RNA PCR were 88.2% and 0.707, respectively. For the diagnosis of AHA, the sensitivity and specificity of IgM anti-HAV were 90.7% and 100%, respectively, when an "equivocal" result was regarded as positive; and 79.1% and 100%, respectively, when an "equivocal" result was regarded as negative. The sensitivity and specificity of HAV RNA PCR were 81.4% and 100%, respectively. All four patients with negative IgM anti-HAV and positive HAV RNA PCR results and all four patients with equivocal IgM anti-HAV RNA and positive HAV RNA PCR results were eventually diagnosed with AHA. The qualitative HAV RNA PCR test has an equivalent diagnostic accuracy for AHA compared to IgM anti-HAV and may be more sensitive during the window period.

UR - http://www.scopus.com/inward/record.url?scp=84879478904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879478904&partnerID=8YFLogxK

U2 - 10.3350/cmh.2012.18.4.397

DO - 10.3350/cmh.2012.18.4.397

M3 - Article

VL - 18

SP - 397

EP - 403

JO - Clinical and molecular hepatology

JF - Clinical and molecular hepatology

SN - 2287-2728

IS - 4

ER -