Multiplex real-time PCR assay for Legionella species

Seung Min Kim, Yoojung Jeong, Jang Wook Sohn, Ja Kim Min

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Legionella pneumophila serogroup 1 (sg1) accounts for the majority of infections in humans, but other Legionella species are also associated with human disease. In this study, a new SYBR Green I-based multiplex real-time PCR assay in a single reaction was developed to allow the rapid detection and differentiation of Legionella species by targeting specific gene sequences. Candidate target genes were selected, and primer sets were designed by referring to comparative genomic hybridization data of Legionella species. The Legionella species-specific groES primer set successfully detected all 30 Legionella strains tested. The xcpX and rfbA primers specifically detected L. pneumophila sg1-15 and L. pneumophila sg1, respectively. In addition, this assay was validated by testing clinical samples and isolates. In conclusion, this novel multiplex real-time PCR assay might be a useful diagnostic tool for the rapid detection and differentiation of Legionella species in both clinical and epidemiological studies.

Original languageEnglish
Pages (from-to)414-419
Number of pages6
JournalMolecular and Cellular Probes
Volume29
Issue number6
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Legionella
Multiplex Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Legionella pneumophila
Comparative Genomic Hybridization
Gene Targeting
Epidemiologic Studies
Infection
Genes
Serogroup

Keywords

  • Legionella species
  • Legionnaires' disease
  • Multiplex
  • Real-time PCR

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Multiplex real-time PCR assay for Legionella species. / Kim, Seung Min; Jeong, Yoojung; Sohn, Jang Wook; Min, Ja Kim.

In: Molecular and Cellular Probes, Vol. 29, No. 6, 01.12.2015, p. 414-419.

Research output: Contribution to journalArticle

Kim, Seung Min ; Jeong, Yoojung ; Sohn, Jang Wook ; Min, Ja Kim. / Multiplex real-time PCR assay for Legionella species. In: Molecular and Cellular Probes. 2015 ; Vol. 29, No. 6. pp. 414-419.
@article{31f2fa1108ec4740b2559acd5b0f4bbb,
title = "Multiplex real-time PCR assay for Legionella species",
abstract = "Legionella pneumophila serogroup 1 (sg1) accounts for the majority of infections in humans, but other Legionella species are also associated with human disease. In this study, a new SYBR Green I-based multiplex real-time PCR assay in a single reaction was developed to allow the rapid detection and differentiation of Legionella species by targeting specific gene sequences. Candidate target genes were selected, and primer sets were designed by referring to comparative genomic hybridization data of Legionella species. The Legionella species-specific groES primer set successfully detected all 30 Legionella strains tested. The xcpX and rfbA primers specifically detected L. pneumophila sg1-15 and L. pneumophila sg1, respectively. In addition, this assay was validated by testing clinical samples and isolates. In conclusion, this novel multiplex real-time PCR assay might be a useful diagnostic tool for the rapid detection and differentiation of Legionella species in both clinical and epidemiological studies.",
keywords = "Legionella species, Legionnaires' disease, Multiplex, Real-time PCR",
author = "Kim, {Seung Min} and Yoojung Jeong and Sohn, {Jang Wook} and Min, {Ja Kim}",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.mcp.2015.08.004",
language = "English",
volume = "29",
pages = "414--419",
journal = "Molecular and Cellular Probes",
issn = "0890-8508",
publisher = "Academic Press Inc.",
number = "6",

}

TY - JOUR

T1 - Multiplex real-time PCR assay for Legionella species

AU - Kim, Seung Min

AU - Jeong, Yoojung

AU - Sohn, Jang Wook

AU - Min, Ja Kim

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Legionella pneumophila serogroup 1 (sg1) accounts for the majority of infections in humans, but other Legionella species are also associated with human disease. In this study, a new SYBR Green I-based multiplex real-time PCR assay in a single reaction was developed to allow the rapid detection and differentiation of Legionella species by targeting specific gene sequences. Candidate target genes were selected, and primer sets were designed by referring to comparative genomic hybridization data of Legionella species. The Legionella species-specific groES primer set successfully detected all 30 Legionella strains tested. The xcpX and rfbA primers specifically detected L. pneumophila sg1-15 and L. pneumophila sg1, respectively. In addition, this assay was validated by testing clinical samples and isolates. In conclusion, this novel multiplex real-time PCR assay might be a useful diagnostic tool for the rapid detection and differentiation of Legionella species in both clinical and epidemiological studies.

AB - Legionella pneumophila serogroup 1 (sg1) accounts for the majority of infections in humans, but other Legionella species are also associated with human disease. In this study, a new SYBR Green I-based multiplex real-time PCR assay in a single reaction was developed to allow the rapid detection and differentiation of Legionella species by targeting specific gene sequences. Candidate target genes were selected, and primer sets were designed by referring to comparative genomic hybridization data of Legionella species. The Legionella species-specific groES primer set successfully detected all 30 Legionella strains tested. The xcpX and rfbA primers specifically detected L. pneumophila sg1-15 and L. pneumophila sg1, respectively. In addition, this assay was validated by testing clinical samples and isolates. In conclusion, this novel multiplex real-time PCR assay might be a useful diagnostic tool for the rapid detection and differentiation of Legionella species in both clinical and epidemiological studies.

KW - Legionella species

KW - Legionnaires' disease

KW - Multiplex

KW - Real-time PCR

UR - http://www.scopus.com/inward/record.url?scp=84951574314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951574314&partnerID=8YFLogxK

U2 - 10.1016/j.mcp.2015.08.004

DO - 10.1016/j.mcp.2015.08.004

M3 - Article

C2 - 26327358

AN - SCOPUS:84951574314

VL - 29

SP - 414

EP - 419

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

SN - 0890-8508

IS - 6

ER -