Multiplex real-time PCR assay for Legionella species

Seung Min Kim, Yoojung Jeong, Jang Wook Sohn, Ja Kim Min

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Legionella pneumophila serogroup 1 (sg1) accounts for the majority of infections in humans, but other Legionella species are also associated with human disease. In this study, a new SYBR Green I-based multiplex real-time PCR assay in a single reaction was developed to allow the rapid detection and differentiation of Legionella species by targeting specific gene sequences. Candidate target genes were selected, and primer sets were designed by referring to comparative genomic hybridization data of Legionella species. The Legionella species-specific groES primer set successfully detected all 30 Legionella strains tested. The xcpX and rfbA primers specifically detected L. pneumophila sg1-15 and L. pneumophila sg1, respectively. In addition, this assay was validated by testing clinical samples and isolates. In conclusion, this novel multiplex real-time PCR assay might be a useful diagnostic tool for the rapid detection and differentiation of Legionella species in both clinical and epidemiological studies.

Original languageEnglish
Pages (from-to)414-419
Number of pages6
JournalMolecular and Cellular Probes
Volume29
Issue number6
DOIs
Publication statusPublished - 2015 Dec 1

Keywords

  • Legionella species
  • Legionnaires' disease
  • Multiplex
  • Real-time PCR

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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