Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence Decoding in Hydrogel

Woongsun Choi, Eunhye Park, Seojin Bae, Kyung Hak Choi, Sangeun Han, Kuk Hui Son, Do Young Lee, Il Joo Cho, Hyejeong Seong, Kyo Seon Hwang, Jwa Min Nam, Jungkyu Choi, Hyojin Lee, Nakwon Choi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Single nucleotide polymorphisms (SNPs) that can alter phenotypes of individuals play a pivotal role in disease development and, more importantly, responses to therapy. However, SNP genotyping has been challenging due to the similarity of SNP alleles and their low concentration in biological samples. Sequence-specific nanoparticle with interpretative toehold-mediated sequence decoding in hydrogel (SWITCH) for multiplex SNP genotyping is presented. The encoding with gold nanoparticle probes transduces each SNP target to ≈1000 invaders with prominently different sequences between wild and mutant types, featuring polymerase chain reaction (PCR)-free amplification. Subsequently, the toehold-mediated DNA replacement in hydrogel microparticles decodes the invaders via SNP-specific fluorescence signals. The 4-plex detection of the warfarin-associated SNP targets spiked in commercially validated human serum (S1-100ML, Merck) is successfully demonstrated with excellent specificity. This work is the first technology development presenting PCR-free, multiplex SNP genotyping with a single reporting fluorophore, to the best of knowledge.

Original languageEnglish
Article number2105538
JournalSmall
Volume18
Issue number8
DOIs
Publication statusPublished - 2022 Feb 24

Keywords

  • gold nanoparticles
  • hydrogel microparticles
  • magnetic microparticles
  • multiplex single nucleotide polymorphism genotyping
  • sandwich assays
  • toehold-mediated DNA displacement
  • warfarin

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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