Multiplexed detection of oligonucleotides with biobarcoded gold nanoparticle probes

Jae Seung Lee

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Applications in a variety of fields rely on the high-throughput ultrasensitive and multiplexed detection of oligonucleotides. However, the conventional microarray-based techniques that employ fluorescent dyes are hampered by several limitations; they require target amplification, fluorophore labeling, and complicated instrumentation, while the fluorophore-labeled species themselves exhibit slow binding kinetics, photo-bleaching effects, and overlapping spectral profiles. Among the emerging nanomaterials that are being used to solve these problems, oligonucleotide-gold nanoparticle conjugates (Oligo-Au NPs) have recently been highlighted due to their unique chemical and physical properties. In this chapter, a detection scheme for oligonucleotides that utilize Oligo-Au NPs is evaluated with multiple oligonucleotide targets. This scheme takes advantage of the sharp melting transitions, intense optical properties, catalytic properties, enhanced binding properties, and the programmable assembly/disassembly of Oligo-Au NPs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages17-31
Number of pages15
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
Volume726
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Biomolecule
  • Detection
  • DNA
  • Gold nanoparticle
  • Multiplexing
  • Oligonucleotide
  • RNA
  • Sensing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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