Multiplexed post-experimental monoisotopic mass refinement (mPE-MMR) to increase sensitivity and accuracy in peptide identifications from tandem mass spectra of cofragmentation

Inamul Hasan Madar, Seung Ik Ko, Hokeun Kim, Dong Gi Mun, Sangtae Kim, Richard D. Smith, Sang-Won Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mass spectrometry (MS)-based proteomics, which uses high-resolution hybrid mass spectrometers such as the quadrupole-orbitrap mass spectrometer, can yield tens of thousands of tandem mass (MS/MS) spectra of high resolution during a routine bottom-up experiment. Despite being a fundamental and key step in MS-based proteomics, the accurate determination and assignment of precursor monoisotopic masses to the MS/MS spectra remains difficult. The difficulties stem from imperfect isotopic envelopes of precursor ions, inaccurate charge states for precursor ions, and cofragmentation. We describe a composite method of utilizing MS data to assign accurate monoisotopic masses to MS/MS spectra, including those subject to cofragmentation. The method, "multiplexed post-experiment monoisotopic mass refinement" (mPE-MMR), consists of the following: multiplexing of precursor masses to assign multiple monoisotopic masses of cofragmented peptides to the corresponding multiplexed MS/MS spectra, multiplexing of charge states to assign correct charges to the precursor ions of MS/ MS spectra with no charge information, and mass correction for inaccurate monoisotopic peak picking. When combined with MS-GF+, a database search algorithm based on fragment mass difference, mPE-MMR effectively increases both sensitivity and accuracy in peptide identification from complex high-throughput proteomics data compared to conventional methods.

Original languageEnglish
Pages (from-to)1244-1253
Number of pages10
JournalAnalytical Chemistry
Volume89
Issue number2
DOIs
Publication statusPublished - 2017 Jan 1

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Mass spectrometry
Peptides
Mass spectrometers
Ions
Multiplexing
Experiments
Throughput

ASJC Scopus subject areas

  • Analytical Chemistry

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Multiplexed post-experimental monoisotopic mass refinement (mPE-MMR) to increase sensitivity and accuracy in peptide identifications from tandem mass spectra of cofragmentation. / Madar, Inamul Hasan; Ko, Seung Ik; Kim, Hokeun; Mun, Dong Gi; Kim, Sangtae; Smith, Richard D.; Lee, Sang-Won.

In: Analytical Chemistry, Vol. 89, No. 2, 01.01.2017, p. 1244-1253.

Research output: Contribution to journalArticle

Madar, Inamul Hasan ; Ko, Seung Ik ; Kim, Hokeun ; Mun, Dong Gi ; Kim, Sangtae ; Smith, Richard D. ; Lee, Sang-Won. / Multiplexed post-experimental monoisotopic mass refinement (mPE-MMR) to increase sensitivity and accuracy in peptide identifications from tandem mass spectra of cofragmentation. In: Analytical Chemistry. 2017 ; Vol. 89, No. 2. pp. 1244-1253.
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