Abstract
Liquid biopsy has been arising to substitute tissue biopsy which has several significant drawbacks. microRNAs (miRNAs) were used as the biomarkers for various diseases including cancers, but conventional diagnosis methods suffered from high cost, false-negative results, or low sensitivity. Here, we developed the signal amplification method for multiplexed miRNA diagnosis method using hydrogel microparticles by modifying hybridization chain reaction (HCR) to hydrogel microparticle-based assay. By doing so, we succeed to amplify fluorescent signal intensity (and thus sensitivity for miRNA) by dozens of times. We also demonstrated the difference between the normal and the patients in specific miRNA profile from urinary exosomal miRNAs.
| Original language | English |
|---|---|
| Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2299-2300 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781510897571 |
| Publication status | Published - 2018 Jan 1 |
| Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 2018 Nov 11 → 2018 Nov 15 |
Publication series
| Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Volume | 4 |
Conference
| Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Country | Taiwan, Province of China |
| City | Kaohsiung |
| Period | 18/11/11 → 18/11/15 |
Keywords
- Hydrogel microparticle
- Liquid biopsy
- miRNA
- Prostate cancer
ASJC Scopus subject areas
- Chemistry(all)
- Bioengineering
- Chemical Engineering (miscellaneous)
- Control and Systems Engineering
Cite this
Kim, J., Bong, K. W., & Choi, N. (2018). Multiplexed signal amplification assay for microRNA using hydrogel microparticles. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 2299-2300). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 4). Chemical and Biological Microsystems Society.