Multiplexed signal amplification assay for microRNA using hydrogel microparticles

Junbeom Kim, Ki Wan Bong, Nakwon Choi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Liquid biopsy has been arising to substitute tissue biopsy which has several significant drawbacks. microRNAs (miRNAs) were used as the biomarkers for various diseases including cancers, but conventional diagnosis methods suffered from high cost, false-negative results, or low sensitivity. Here, we developed the signal amplification method for multiplexed miRNA diagnosis method using hydrogel microparticles by modifying hybridization chain reaction (HCR) to hydrogel microparticle-based assay. By doing so, we succeed to amplify fluorescent signal intensity (and thus sensitivity for miRNA) by dozens of times. We also demonstrated the difference between the normal and the patients in specific miRNA profile from urinary exosomal miRNAs.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages2299-2300
Number of pages2
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period18/11/1118/11/15

Keywords

  • Hydrogel microparticle
  • Liquid biopsy
  • miRNA
  • Prostate cancer

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

Cite this

Kim, J., Bong, K. W., & Choi, N. (2018). Multiplexed signal amplification assay for microRNA using hydrogel microparticles. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 2299-2300). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 4). Chemical and Biological Microsystems Society.