TY - GEN
T1 - Multiplexed signal amplification assay for microRNA using hydrogel microparticles
AU - Kim, Junbeom
AU - Bong, Ki Wan
AU - Choi, Nakwon
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Liquid biopsy has been arising to substitute tissue biopsy which has several significant drawbacks. microRNAs (miRNAs) were used as the biomarkers for various diseases including cancers, but conventional diagnosis methods suffered from high cost, false-negative results, or low sensitivity. Here, we developed the signal amplification method for multiplexed miRNA diagnosis method using hydrogel microparticles by modifying hybridization chain reaction (HCR) to hydrogel microparticle-based assay. By doing so, we succeed to amplify fluorescent signal intensity (and thus sensitivity for miRNA) by dozens of times. We also demonstrated the difference between the normal and the patients in specific miRNA profile from urinary exosomal miRNAs.
AB - Liquid biopsy has been arising to substitute tissue biopsy which has several significant drawbacks. microRNAs (miRNAs) were used as the biomarkers for various diseases including cancers, but conventional diagnosis methods suffered from high cost, false-negative results, or low sensitivity. Here, we developed the signal amplification method for multiplexed miRNA diagnosis method using hydrogel microparticles by modifying hybridization chain reaction (HCR) to hydrogel microparticle-based assay. By doing so, we succeed to amplify fluorescent signal intensity (and thus sensitivity for miRNA) by dozens of times. We also demonstrated the difference between the normal and the patients in specific miRNA profile from urinary exosomal miRNAs.
KW - Hydrogel microparticle
KW - Liquid biopsy
KW - miRNA
KW - Prostate cancer
UR - http://www.scopus.com/inward/record.url?scp=85079814797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079814797&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079814797
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 2299
EP - 2300
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -