Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography

Hyeong Soo Nam, Woo Jae Kang, Min Woo Lee, Joon Woo Song, Jin Won Kim, Wang Yuhl Oh, Hongki Yoo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The pathophysiological progression of chronic diseases, including atherosclerosis and cancer, is closely related to compositional changes in biological tissues containing endogenous fluorophores such as collagen, elastin, and NADH, which exhibit strong autofluorescence under ultraviolet excitation. Fluorescence lifetime imaging (FLIm) provides robust detection of the compositional changes by measuring fluorescence lifetime, which is an inherent property of a fluorophore. In this paper, we present a dual-modality system combining a multispectral analog-mean-delay (AMD) FLIm and a high-speed swept-source optical coherence tomography (OCT) to simultaneously visualize the cross-sectional morphology and biochemical compositional information of a biological tissue. Experiments using standard fluorescent solutions showed that the fluorescence lifetime could be measured with a precision of less than 40 psec using the multispectral AMD-FLIm without averaging. In addition, we performed ex vivo imaging on rabbit iliac normal-looking and atherosclerotic specimens to demonstrate the feasibility of the combined FLIm-OCT system for atherosclerosis imaging. We expect that the combined FLIm-OCT will be a promising nextgeneration imaging technique for diagnosing atherosclerosis and cancer due to the advantages of the proposed label-free high-precision multispectral lifetime measurement.

Original languageEnglish
Article number#315153
Pages (from-to)1930-1947
Number of pages18
JournalBiomedical Optics Express
Volume9
Issue number4
DOIs
Publication statusPublished - 2018 Apr 1

Fingerprint

Optical Imaging
Optical Coherence Tomography
tomography
analogs
life (durability)
fluorescence
Atherosclerosis
arteriosclerosis
Fluorescence
Elastin
NAD
cancer
elastin
Neoplasms
Chronic Disease
Collagen
rabbits
Rabbits
collagens
progressions

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography. / Nam, Hyeong Soo; Kang, Woo Jae; Lee, Min Woo; Song, Joon Woo; Kim, Jin Won; Oh, Wang Yuhl; Yoo, Hongki.

In: Biomedical Optics Express, Vol. 9, No. 4, #315153, 01.04.2018, p. 1930-1947.

Research output: Contribution to journalArticle

Nam, Hyeong Soo ; Kang, Woo Jae ; Lee, Min Woo ; Song, Joon Woo ; Kim, Jin Won ; Oh, Wang Yuhl ; Yoo, Hongki. / Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography. In: Biomedical Optics Express. 2018 ; Vol. 9, No. 4. pp. 1930-1947.
@article{6c7d2146a88a4f1f941dd1bd17558065,
title = "Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography",
abstract = "The pathophysiological progression of chronic diseases, including atherosclerosis and cancer, is closely related to compositional changes in biological tissues containing endogenous fluorophores such as collagen, elastin, and NADH, which exhibit strong autofluorescence under ultraviolet excitation. Fluorescence lifetime imaging (FLIm) provides robust detection of the compositional changes by measuring fluorescence lifetime, which is an inherent property of a fluorophore. In this paper, we present a dual-modality system combining a multispectral analog-mean-delay (AMD) FLIm and a high-speed swept-source optical coherence tomography (OCT) to simultaneously visualize the cross-sectional morphology and biochemical compositional information of a biological tissue. Experiments using standard fluorescent solutions showed that the fluorescence lifetime could be measured with a precision of less than 40 psec using the multispectral AMD-FLIm without averaging. In addition, we performed ex vivo imaging on rabbit iliac normal-looking and atherosclerotic specimens to demonstrate the feasibility of the combined FLIm-OCT system for atherosclerosis imaging. We expect that the combined FLIm-OCT will be a promising nextgeneration imaging technique for diagnosing atherosclerosis and cancer due to the advantages of the proposed label-free high-precision multispectral lifetime measurement.",
author = "Nam, {Hyeong Soo} and Kang, {Woo Jae} and Lee, {Min Woo} and Song, {Joon Woo} and Kim, {Jin Won} and Oh, {Wang Yuhl} and Hongki Yoo",
year = "2018",
month = "4",
day = "1",
doi = "10.1364/BOE.9.001930",
language = "English",
volume = "9",
pages = "1930--1947",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "4",

}

TY - JOUR

T1 - Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography

AU - Nam, Hyeong Soo

AU - Kang, Woo Jae

AU - Lee, Min Woo

AU - Song, Joon Woo

AU - Kim, Jin Won

AU - Oh, Wang Yuhl

AU - Yoo, Hongki

PY - 2018/4/1

Y1 - 2018/4/1

N2 - The pathophysiological progression of chronic diseases, including atherosclerosis and cancer, is closely related to compositional changes in biological tissues containing endogenous fluorophores such as collagen, elastin, and NADH, which exhibit strong autofluorescence under ultraviolet excitation. Fluorescence lifetime imaging (FLIm) provides robust detection of the compositional changes by measuring fluorescence lifetime, which is an inherent property of a fluorophore. In this paper, we present a dual-modality system combining a multispectral analog-mean-delay (AMD) FLIm and a high-speed swept-source optical coherence tomography (OCT) to simultaneously visualize the cross-sectional morphology and biochemical compositional information of a biological tissue. Experiments using standard fluorescent solutions showed that the fluorescence lifetime could be measured with a precision of less than 40 psec using the multispectral AMD-FLIm without averaging. In addition, we performed ex vivo imaging on rabbit iliac normal-looking and atherosclerotic specimens to demonstrate the feasibility of the combined FLIm-OCT system for atherosclerosis imaging. We expect that the combined FLIm-OCT will be a promising nextgeneration imaging technique for diagnosing atherosclerosis and cancer due to the advantages of the proposed label-free high-precision multispectral lifetime measurement.

AB - The pathophysiological progression of chronic diseases, including atherosclerosis and cancer, is closely related to compositional changes in biological tissues containing endogenous fluorophores such as collagen, elastin, and NADH, which exhibit strong autofluorescence under ultraviolet excitation. Fluorescence lifetime imaging (FLIm) provides robust detection of the compositional changes by measuring fluorescence lifetime, which is an inherent property of a fluorophore. In this paper, we present a dual-modality system combining a multispectral analog-mean-delay (AMD) FLIm and a high-speed swept-source optical coherence tomography (OCT) to simultaneously visualize the cross-sectional morphology and biochemical compositional information of a biological tissue. Experiments using standard fluorescent solutions showed that the fluorescence lifetime could be measured with a precision of less than 40 psec using the multispectral AMD-FLIm without averaging. In addition, we performed ex vivo imaging on rabbit iliac normal-looking and atherosclerotic specimens to demonstrate the feasibility of the combined FLIm-OCT system for atherosclerosis imaging. We expect that the combined FLIm-OCT will be a promising nextgeneration imaging technique for diagnosing atherosclerosis and cancer due to the advantages of the proposed label-free high-precision multispectral lifetime measurement.

UR - http://www.scopus.com/inward/record.url?scp=85044943395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044943395&partnerID=8YFLogxK

U2 - 10.1364/BOE.9.001930

DO - 10.1364/BOE.9.001930

M3 - Article

AN - SCOPUS:85044943395

VL - 9

SP - 1930

EP - 1947

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 4

M1 - #315153

ER -