Muricauda ochracea sp. Nov., isolated from a tidal flat in the republic of korea

Dongjun Kim, Yeonjae Yoo, Jong Seong Khim, Dongmin Yang, Duleepa Pathiraja, In Geol Choi, Jae Jin Kim

Research output: Contribution to journalArticlepeer-review

Abstract

A yellowish-brown-coloured bacterium, designated strain JGD-17T, was isolated from a tidal flat of Janggu-do, Garorim bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-flagellated and long-rod-shaped. Growth was observed at 20–45 °C (optimum, 25–30 °C), at pH 6.0–10.0 (9.0) and with 1–5% (w/v) NaCl (1–3%). Results of 16S rRNA gene sequence analysis indicated that strain JGD-17T was closely related to Muricauda nanhaiensis SM1704T (96.1%), Muricauda olearia CL-SS4T (95.0%), Muricauda beolgyonensis BB-My12T (94.9%), Muricauda marina H19-56T (94.7%) and Muricauda indica 3PC125-7T (94.5%). The ranges of values for the average nucleotide identity and digital DNA–DNA hybridiza-tion analyses with related strains were 71.3–74.1% and 16.9–18.2%. The genomic DNA G+C content was 41.1 mol%. Phylogenetic analysis using the neighbour-joining method showed that strain JGD-17T formed a clade with Muricauda nanhaiensis SM1704T, Muricauda lutaonensis CC-HSB-11T, Muricauda lutea CSW06T and Muricauda pacifica SM027T. The major fatty acids were iso-C15:0 (26.9%), iso-C15:1 G (19.5%) and iso-C17:0 3-OH (12.7%). The predominant respiratory quinone was menaquinone-6. The polar lipids were phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain JGD-17T represents a novel species within the genus Muricauda, for which the name Muricauda ochracea sp. nov. is proposed. The type strain is JGD-17T (=KCTC 72732T=KACC 21486T=JCM 33817T).

Original languageEnglish
Article number004312
Pages (from-to)4555-4561
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number8
DOIs
Publication statusPublished - 2020

Keywords

  • Marine bacteria
  • Muricauda
  • Polyphasic approach
  • Tidal flats

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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