Muscle regeneration by adipose tissue-derived adult stem cells attached to injectable PLGA spheres

Mi Jung Kim, Yu Suk Choi, Seung Hye Yang, Hea Nam Hong, Sung Woo Cho, Sang Myun Cha, Jhang Ho Pak, Chan Wha Kim, Seog Woon Kwon, Chan Jeoung Park

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

The use of adult stem cells for cell-based tissue engineering and regeneration strategies represents a promising approach for skeletal muscle repair. We have evaluated the combination of adipose tissue-derived adult stem cells (ADSCs) obtained from autologous liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for muscle regeneration. ADSCs attached to PLGA spheres and PLGA spheres alone were cultured in myogenic medium for 21 days and injected subcutaneously into the necks of nude mice. After 30 and 60 days, the mice were sacrificed, and newly formed tissues were analyzed by immunostaining, H and E staining, and RT-PCR. We found that ADSCs attached to PLGA spheres, but not PLGA spheres alone, were able to generate muscle tissue. These findings suggest that ADSCs and PLGA spheres are useful materials for muscle tissue engineering and that their combination can be used in clinical settings for muscle regeneration.

Original languageEnglish
Pages (from-to)386-392
Number of pages7
JournalBiochemical and biophysical research communications
Volume348
Issue number2
DOIs
Publication statusPublished - 2006 Sep 22

Keywords

  • Adipose tissue-derived adult stem cells
  • Injectable scaffold
  • Muscle regeneration
  • Myogenic differentiation
  • PLGA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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