Mussel adhesive Protein-conjugated Vitronectin (fp-151-VT) Induces Anti-inflammatory Activity on LPS-stimulated Macrophages and UVB-irradiated Keratinocytes

Jung Mo Ahn, Jun Sik Lee, Seul Gee Um, Beom Seop Rho, Ki Beom Lee, Sung Gil Park, Ho jin Kim, Yoonjin Lee, Young Min Chi, Ye Eun Yoon, Sun Hyo Jo, Mi Eun Kim, Kyung Bae Pi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Skin inflammation and dermal injuries are a major clinical problem because current therapies are limited to treating established scars, and there is a poor understanding of healing mechanisms. Mussel adhesive proteins (MAPs) have great potential in many tissue engineering and biomedical applications. It has been successfully demonstrated that the redesigned hybrid type MAP (fp-151) can be utilized as a promising adhesive biomaterial. The aim of this study was to develop a novel recombinant protein using fp-151 and vitronectin (VT) and to elucidate the anti-inflammatory effects of this recombinant protein on macrophages and keratinocytes. Methods: Lipopolysaccharide (LPS) was used to stimulate macrophages and UVB was used to stimulate keratinocytes. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were analyzed by Western Blot. Inflammatory cytokines and NO and ROS production were analyzed. Result: In macrophages stimulated by LPS, expression of the inflammatory factors iNOS, COX-2, and NO production increased, while the r-fp-151-VT-treated groups had suppressed expression of iNOS, COX-2, and NO production in a dose-dependent manner. In addition, keratinocytes stimulated by UVB and treated with r-fp-151-VT had reduced expression of iNOS and COX-2. Interestingly, in UVB-irradiated keratinocytes, inflammatory cytokines, such as interleukin (IL)-1b, IL-6, and tumor necrosis factor (TNF)-a, were significantly reduced by r-fp-151-VT treatment. Conclusions: These results suggest that the anti-inflammatory activity of r-fp-151-VT was more effective in keratinocytes, suggesting that it can be used as a therapeutic agent to treat skin inflammation.

Original languageEnglish
JournalImmunological Investigations
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Vitronectin
Keratinocytes
Lipopolysaccharides
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Anti-Inflammatory Agents
Macrophages
Recombinant Proteins
Skin
Cytokines
Inflammation
Interleukins
Biocompatible Materials
Tissue Engineering
Adhesives
Cicatrix
Interleukin-6
Tumor Necrosis Factor-alpha
Western Blotting
mussel adhesive protein

Keywords

  • anti-inflammatory activity
  • Mussel adhesive protein
  • Vitronectin

ASJC Scopus subject areas

  • Immunology

Cite this

Mussel adhesive Protein-conjugated Vitronectin (fp-151-VT) Induces Anti-inflammatory Activity on LPS-stimulated Macrophages and UVB-irradiated Keratinocytes. / Ahn, Jung Mo; Lee, Jun Sik; Um, Seul Gee; Rho, Beom Seop; Lee, Ki Beom; Park, Sung Gil; Kim, Ho jin; Lee, Yoonjin; Chi, Young Min; Yoon, Ye Eun; Jo, Sun Hyo; Kim, Mi Eun; Pi, Kyung Bae.

In: Immunological Investigations, 01.01.2018.

Research output: Contribution to journalArticle

Ahn, Jung Mo ; Lee, Jun Sik ; Um, Seul Gee ; Rho, Beom Seop ; Lee, Ki Beom ; Park, Sung Gil ; Kim, Ho jin ; Lee, Yoonjin ; Chi, Young Min ; Yoon, Ye Eun ; Jo, Sun Hyo ; Kim, Mi Eun ; Pi, Kyung Bae. / Mussel adhesive Protein-conjugated Vitronectin (fp-151-VT) Induces Anti-inflammatory Activity on LPS-stimulated Macrophages and UVB-irradiated Keratinocytes. In: Immunological Investigations. 2018.
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title = "Mussel adhesive Protein-conjugated Vitronectin (fp-151-VT) Induces Anti-inflammatory Activity on LPS-stimulated Macrophages and UVB-irradiated Keratinocytes",
abstract = "Background: Skin inflammation and dermal injuries are a major clinical problem because current therapies are limited to treating established scars, and there is a poor understanding of healing mechanisms. Mussel adhesive proteins (MAPs) have great potential in many tissue engineering and biomedical applications. It has been successfully demonstrated that the redesigned hybrid type MAP (fp-151) can be utilized as a promising adhesive biomaterial. The aim of this study was to develop a novel recombinant protein using fp-151 and vitronectin (VT) and to elucidate the anti-inflammatory effects of this recombinant protein on macrophages and keratinocytes. Methods: Lipopolysaccharide (LPS) was used to stimulate macrophages and UVB was used to stimulate keratinocytes. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were analyzed by Western Blot. Inflammatory cytokines and NO and ROS production were analyzed. Result: In macrophages stimulated by LPS, expression of the inflammatory factors iNOS, COX-2, and NO production increased, while the r-fp-151-VT-treated groups had suppressed expression of iNOS, COX-2, and NO production in a dose-dependent manner. In addition, keratinocytes stimulated by UVB and treated with r-fp-151-VT had reduced expression of iNOS and COX-2. Interestingly, in UVB-irradiated keratinocytes, inflammatory cytokines, such as interleukin (IL)-1b, IL-6, and tumor necrosis factor (TNF)-a, were significantly reduced by r-fp-151-VT treatment. Conclusions: These results suggest that the anti-inflammatory activity of r-fp-151-VT was more effective in keratinocytes, suggesting that it can be used as a therapeutic agent to treat skin inflammation.",
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author = "Ahn, {Jung Mo} and Lee, {Jun Sik} and Um, {Seul Gee} and Rho, {Beom Seop} and Lee, {Ki Beom} and Park, {Sung Gil} and Kim, {Ho jin} and Yoonjin Lee and Chi, {Young Min} and Yoon, {Ye Eun} and Jo, {Sun Hyo} and Kim, {Mi Eun} and Pi, {Kyung Bae}",
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T1 - Mussel adhesive Protein-conjugated Vitronectin (fp-151-VT) Induces Anti-inflammatory Activity on LPS-stimulated Macrophages and UVB-irradiated Keratinocytes

AU - Ahn, Jung Mo

AU - Lee, Jun Sik

AU - Um, Seul Gee

AU - Rho, Beom Seop

AU - Lee, Ki Beom

AU - Park, Sung Gil

AU - Kim, Ho jin

AU - Lee, Yoonjin

AU - Chi, Young Min

AU - Yoon, Ye Eun

AU - Jo, Sun Hyo

AU - Kim, Mi Eun

AU - Pi, Kyung Bae

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Skin inflammation and dermal injuries are a major clinical problem because current therapies are limited to treating established scars, and there is a poor understanding of healing mechanisms. Mussel adhesive proteins (MAPs) have great potential in many tissue engineering and biomedical applications. It has been successfully demonstrated that the redesigned hybrid type MAP (fp-151) can be utilized as a promising adhesive biomaterial. The aim of this study was to develop a novel recombinant protein using fp-151 and vitronectin (VT) and to elucidate the anti-inflammatory effects of this recombinant protein on macrophages and keratinocytes. Methods: Lipopolysaccharide (LPS) was used to stimulate macrophages and UVB was used to stimulate keratinocytes. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were analyzed by Western Blot. Inflammatory cytokines and NO and ROS production were analyzed. Result: In macrophages stimulated by LPS, expression of the inflammatory factors iNOS, COX-2, and NO production increased, while the r-fp-151-VT-treated groups had suppressed expression of iNOS, COX-2, and NO production in a dose-dependent manner. In addition, keratinocytes stimulated by UVB and treated with r-fp-151-VT had reduced expression of iNOS and COX-2. Interestingly, in UVB-irradiated keratinocytes, inflammatory cytokines, such as interleukin (IL)-1b, IL-6, and tumor necrosis factor (TNF)-a, were significantly reduced by r-fp-151-VT treatment. Conclusions: These results suggest that the anti-inflammatory activity of r-fp-151-VT was more effective in keratinocytes, suggesting that it can be used as a therapeutic agent to treat skin inflammation.

AB - Background: Skin inflammation and dermal injuries are a major clinical problem because current therapies are limited to treating established scars, and there is a poor understanding of healing mechanisms. Mussel adhesive proteins (MAPs) have great potential in many tissue engineering and biomedical applications. It has been successfully demonstrated that the redesigned hybrid type MAP (fp-151) can be utilized as a promising adhesive biomaterial. The aim of this study was to develop a novel recombinant protein using fp-151 and vitronectin (VT) and to elucidate the anti-inflammatory effects of this recombinant protein on macrophages and keratinocytes. Methods: Lipopolysaccharide (LPS) was used to stimulate macrophages and UVB was used to stimulate keratinocytes. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were analyzed by Western Blot. Inflammatory cytokines and NO and ROS production were analyzed. Result: In macrophages stimulated by LPS, expression of the inflammatory factors iNOS, COX-2, and NO production increased, while the r-fp-151-VT-treated groups had suppressed expression of iNOS, COX-2, and NO production in a dose-dependent manner. In addition, keratinocytes stimulated by UVB and treated with r-fp-151-VT had reduced expression of iNOS and COX-2. Interestingly, in UVB-irradiated keratinocytes, inflammatory cytokines, such as interleukin (IL)-1b, IL-6, and tumor necrosis factor (TNF)-a, were significantly reduced by r-fp-151-VT treatment. Conclusions: These results suggest that the anti-inflammatory activity of r-fp-151-VT was more effective in keratinocytes, suggesting that it can be used as a therapeutic agent to treat skin inflammation.

KW - anti-inflammatory activity

KW - Mussel adhesive protein

KW - Vitronectin

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