Abstract
We examined the in vitro mutagenic and antimutagenic effects of hexane-extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities ≤400 g/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.
| Original language | English |
|---|---|
| Pages (from-to) | 396-400 |
| Number of pages | 5 |
| Journal | Korean Journal of Food Science and Technology |
| Volume | 43 |
| Issue number | 3 |
| Publication status | Published - 2011 Jun 1 |
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Keywords
- Ames test
- Antimutagenicity
- Hemp seed oil
- Mutagenicity
ASJC Scopus subject areas
- Food Science
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Mutagenic and antimutagenic effects of hemp seed oil evaluated by ames Salmonella testing. / Jeun, Jungae; Cho, Hee Joon; Jun, Hee Jin; Lee, Ji Hae; Jia, Yaoyao; Cho, Kyoung Sang; Kim, Eun Soo; Lee, Sung Joon.
In: Korean Journal of Food Science and Technology, Vol. 43, No. 3, 01.06.2011, p. 396-400.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mutagenic and antimutagenic effects of hemp seed oil evaluated by ames Salmonella testing
AU - Jeun, Jungae
AU - Cho, Hee Joon
AU - Jun, Hee Jin
AU - Lee, Ji Hae
AU - Jia, Yaoyao
AU - Cho, Kyoung Sang
AU - Kim, Eun Soo
AU - Lee, Sung Joon
PY - 2011/6/1
Y1 - 2011/6/1
N2 - We examined the in vitro mutagenic and antimutagenic effects of hexane-extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities ≤400 g/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.
AB - We examined the in vitro mutagenic and antimutagenic effects of hexane-extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities ≤400 g/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.
KW - Ames test
KW - Antimutagenicity
KW - Hemp seed oil
KW - Mutagenicity
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UR - http://www.scopus.com/inward/citedby.url?scp=79960168585&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:79960168585
VL - 43
SP - 396
EP - 400
JO - Korean Journal of Food Science and Technology
JF - Korean Journal of Food Science and Technology
SN - 0367-6293
IS - 3
ER -