Mutation of a positively charged cytoplasmic motif within CD1d results in multiple defects in antigen presentation to NKT cells

Jung Hoon Shin, Ji Young Park, Young Hyun Shin, Hyunji Lee, Yoon Kyung Park, Sundo Jung, Se Ho Park

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

CD1d is an MHC class I-like molecule that presents glycolipid Ags to types I and II NKT cells. The YxxI motif in the cytoplasmic tail of CD1d contributes to its intracellular localization to the endolysosomal compartment and is important for Ag presentation to type I NKT cells. In this study, we identified the 327-329RRR motif in CD1d and showed that it is critical for the control of CD1d intracellular trafficking and Ag presentation. The replacement of the arginines in this motif with alanines resulted in the extensive accumulation of CD1d in lysosomes but did not affect the cell surface expression. The defect in its cellular localization was accompanied by defects in Ag presentation to both type I and type II NKT cells. These results demonstrated that the 327-329RRR motif of CD1d is required for proper cellular distribution of CD1d and optimal Ag presentation to both type I and type II NKT cells.

Original languageEnglish
Pages (from-to)2235-2243
Number of pages9
JournalJournal of Immunology
Volume188
Issue number5
DOIs
Publication statusPublished - 2012 Mar 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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