Mutations in ArgS Arginine-tRNA Synthetase Confer Additional Antibiotic Tolerance Protection to Extended-Spectrum-β-Lactamase-Producing Burkholderia thailandensis

Hyojeong Yi, Jongwook Park, Kwang Hwi Cho, Heenam Stanley Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Highly conserved PenI-type class A β-lactamase in pathogenic members of Burkholderia species can evolve to extended-spectrum β-lactamase (ESBL), which exhibits hydrolytic activity toward third-generation cephalosporins, while losing its activity toward the original penicillin substrates. We describe three single-amino-acid-substitution mutations in the ArgS arginine-tRNA synthetase that confer extra antibiotic tolerance protection to ESBL-producing Burkholderia thailandensis. This pathway can be exploited to evade antibiotic tolerance induction in developing therapeutic measures against Burkholderia species, targeting their essential aminoacyl-tRNA synthetases.

Original languageEnglish
Article numbere02252-19
JournalAntimicrobial Agents and Chemotherapy
Volume64
Issue number6
DOIs
Publication statusPublished - 2020 Jun

Keywords

  • Antibiotic tolerance
  • ArgS
  • Arginine-tRNA synthetase
  • Stringent response

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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