Mutations in the human 5-lipoxygenase gene

Kwang Ho In, Eric S. Silverman, Koichiro Asano, David Beier, Andrew R. Fischer, Tim P. Keith, Kristina Serino, Chandri Yandava, George T. De Sanctis, Jeffrey M. Drazen

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14 Citations (Scopus)

Abstract

Our data demonstrate the presence of a naturally occurring family of alleles in the core promoter of the 5-LO gene, which is characterized by the deletion or addition of consensus Sp1 (-GGGCGG) and Egr-1 (-GCGGGGGCG-) binding motifs. Each of the variant alleles can bind Sp1 and Egr-1 protein, as indicated by EMSA and supershift analysis with nuclear extracts. In addition, preliminary data from CAT reporter assays indicate that these alleles are less effective than the wild-type allele in initiating 5-LO gene expression. Whether patients harboring the various alleles identified herein have different capacities to transcribe the 5-LO gene and the importance of such potential regulation to the clinical expression of 5-LO have yet to be determined.

Original languageEnglish
Pages (from-to)59-69
Number of pages11
JournalClinical Reviews in Allergy and Immunology
Volume17
Issue number1-2
DOIs
Publication statusPublished - 1999 Jan 1
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

In, K. H., Silverman, E. S., Asano, K., Beier, D., Fischer, A. R., Keith, T. P., Serino, K., Yandava, C., De Sanctis, G. T., & Drazen, J. M. (1999). Mutations in the human 5-lipoxygenase gene. Clinical Reviews in Allergy and Immunology, 17(1-2), 59-69. https://doi.org/10.1007/BF02737597