Mutations of the immunoglobulin heavy chain variable region gene in CD99-deficient BJAB cell line

Young Ho Suh, Min Kyung Kim, Young Kee Shin, Seok Hyung Kim, Kwon Ik Oh, Minchan Gil, Min Kyung Kim, Yoon La Choi, Kyeong Cheon Jung, Kyung Mi Lee, Im Soon Lee, Seong Hoe Park

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Hodgkin's disease (HD) is a lymphoid neoplasm characterized by a low frequency of malignant giant tumor cells, known as Hodgkin's and Reed-Sternberg (HRS) cells. Sequence analysis of the immunoglobulin heavy chain hypervariable region (IgH V) genes of HRS cells revealed multiple nucleotide substitutions, indicating somatic mutations, and suggested that HRS cells originate from germinal center B cells or their progeny. We previously reported that CD99-antisense transfected B cell lines led to the generation of cells with a HRS phenotype. Because it is considered that HRS cells in HD carry somatic mutations of the IgH genes, we assume that somatic mutation may take place in the IgH genes of HRS-like cells which do not express CD99. Here we report that CD99 downregulated BJAB cell line has several mutations in IgH V genes. The frequency of mutation was 5.2 × 10-1 mut.bp-1 out of total sequenced cell clones. On the contrary, control vector transfected BJAB cell line or CD99 downregulated IM9 cell line did not show any mutations on single strand conformational polymorphism (SSCP) and sequence analysis. We expect that the analysis of the mutation pattern of the CD99-deficient BJAB cell line might be the basis for the understanding of the molecular and cellular mechanism that regulate somatic mutation and B cell selection.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalMolecules and cells
Volume13
Issue number2
Publication statusPublished - 2002 Apr

Keywords

  • BJAB Cell Line
  • CD99
  • Immunoglobulin Heavy Chain Hypervariable Region Gene
  • Lymphoma
  • Somatic Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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