MYBD employed by HY5 increases anthocyanin accumulation via repression of MYBL2 in Arabidopsis

Nguyen Hoai Nguyen, Chan Young Jeong, Geun Ho Kang, Sang Dong Yoo, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Photomorphogenesis is an essential program in plant development. This process is effected by the balanced cooperation of many factors under light and dark conditions. In a previous study, we showed that MYB hypocotyl elongation-related (MYBH) is involved in cell elongation. To expand our understanding of MYBH function, we performed a yeast two-hybrid assay and identified an MYB-like Domain transcription factor (MYBD). In this study, we investigated the function of MYBD, which is an MYBH homolog involved in anthocyanin accumulation. MYBD expression increased in response to light or cytokinin, and MYBD enhanced anthocyanin biosynthesis via repression of MYBL2, which encodes a transcription factor that has a negative effect on this process. In addition, MYBD binding in vivo to the MYBL2 promoter and the lower level of histone H3K9 acetylation at the upstream region of MYBL2 in MYBD over-expressing plants in comparison with wild-type plants imply that MYBD represses MYBL2 expression via an epigenetic mechanism. HY5 directly binds to the MYBD promoter, which indicates that MYBD acts on HY5-downstream in light- or cytokinin-triggered signaling pathways, leading to anthocyanin accumulation. Our results suggest that, although MYBD and MYBH are homologs, they act in opposite ways during plant photomorphogenesis, and these functions should be examined in further studies. Significance Statement MYB transcription factors are important in the light-cytokinin signaling pathway. Here we show that MYBD plays a positive role in anthocyanin accumulation in response to cytokinin via repression of MYBL2.

Original languageEnglish
Pages (from-to)1192-1205
Number of pages14
JournalPlant Journal
Volume84
Issue number6
DOIs
Publication statusPublished - 2015 Dec 1

Keywords

  • Arabidopsis thaliana
  • MYBD (AT1G70000)
  • MYBL2 (AT1G71030)
  • anthocyanin
  • cytokinin
  • histone acetylation
  • photomorphogenesis

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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