Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare

So Young Lee, Byoung Jun Kim, Hong Kim, Yu Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji Hun Lim, Seung Heon Lee, Chang Ki Kim, Yoon Hoh Kook, Bum Joon Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).

Original languageEnglish
Article number001158
Pages (from-to)3132-3141
Number of pages10
JournalInternational journal of systematic and evolutionary microbiology
Volume66
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1
Externally publishedYes

Fingerprint

Mycobacterium intracellulare
Mycobacterium avium Complex
Mycobacterium
genotype
Genotype
Multilocus Sequence Typing
chimera
DNA
gene
agar
targeting
Mycolic Acids
desorption
ionization
laser
lipid
Mycobacterium avium complex
Essential Genes
phylogenetics
matrix

Keywords

  • Multilocus sequence typing analysis (MLST)
  • Mycobacterium avium complex
  • Mycobacterium intracellulare INT 1 group
  • Mycobacterium paraintracellulare

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare. / Lee, So Young; Kim, Byoung Jun; Kim, Hong; Won, Yu Seop; Jeon, Che Ok; Jeong, Joseph; Lee, Seon Ho; Lim, Ji Hun; Lee, Seung Heon; Kim, Chang Ki; Kook, Yoon Hoh; Kim, Bum Joon.

In: International journal of systematic and evolutionary microbiology, Vol. 66, No. 8, 001158, 01.08.2016, p. 3132-3141.

Research output: Contribution to journalArticle

Lee, SY, Kim, BJ, Kim, H, Won, YS, Jeon, CO, Jeong, J, Lee, SH, Lim, JH, Lee, SH, Kim, CK, Kook, YH & Kim, BJ 2016, 'Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare', International journal of systematic and evolutionary microbiology, vol. 66, no. 8, 001158, pp. 3132-3141. https://doi.org/10.1099/ijsem.0.001158
Lee, So Young ; Kim, Byoung Jun ; Kim, Hong ; Won, Yu Seop ; Jeon, Che Ok ; Jeong, Joseph ; Lee, Seon Ho ; Lim, Ji Hun ; Lee, Seung Heon ; Kim, Chang Ki ; Kook, Yoon Hoh ; Kim, Bum Joon. / Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare. In: International journal of systematic and evolutionary microbiology. 2016 ; Vol. 66, No. 8. pp. 3132-3141.
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