Abstract
Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).
| Original language | English |
|---|---|
| Article number | 001158 |
| Pages (from-to) | 3132-3141 |
| Number of pages | 10 |
| Journal | International journal of systematic and evolutionary microbiology |
| Volume | 66 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2016 Aug 1 |
| Externally published | Yes |
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Keywords
- Multilocus sequence typing analysis (MLST)
- Mycobacterium avium complex
- Mycobacterium intracellulare INT 1 group
- Mycobacterium paraintracellulare
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare. / Lee, So Young; Kim, Byoung Jun; Kim, Hong; Won, Yu Seop; Jeon, Che Ok; Jeong, Joseph; Lee, Seon Ho; Lim, Ji Hun; Lee, Seung Heon; Kim, Chang Ki; Kook, Yoon Hoh; Kim, Bum Joon.
In: International journal of systematic and evolutionary microbiology, Vol. 66, No. 8, 001158, 01.08.2016, p. 3132-3141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare
AU - Lee, So Young
AU - Kim, Byoung Jun
AU - Kim, Hong
AU - Won, Yu Seop
AU - Jeon, Che Ok
AU - Jeong, Joseph
AU - Lee, Seon Ho
AU - Lim, Ji Hun
AU - Lee, Seung Heon
AU - Kim, Chang Ki
AU - Kook, Yoon Hoh
AU - Kim, Bum Joon
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).
AB - Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).
KW - Multilocus sequence typing analysis (MLST)
KW - Mycobacterium avium complex
KW - Mycobacterium intracellulare INT 1 group
KW - Mycobacterium paraintracellulare
UR - http://www.scopus.com/inward/record.url?scp=84988336013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84988336013&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001158
DO - 10.1099/ijsem.0.001158
M3 - Article
VL - 66
SP - 3132
EP - 3141
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 8
M1 - 001158
ER -