Myogenic differentiation of p53- and Rb-deficient immortalized and transformed bovine fibroblasts in response to MyoD

Xun Jin, Joong Seub Lee, Sungwook Kwak, Ji Eun Jung, Tae Kyung Kim, Chenxiong Xu, Zhongshan Hong, Zhehu Li, Sun Myoung Kim, Kwang Youn Whang, Ki Chang Hong, Seungkwon You, Yun Jaie Choi, Hyunggee Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have established in culture a spontaneously immortalized bovine embryonic fibroblast (BEF) cell line that has lost p53 and p16INK4a functions. MyoD is a muscle-specific regulator capable of inducing myogenesis in a number of cell types. When the BEF cells were transduced with MyoD they differentiated efficiently to desmin-positive myofibers in the presence of 2% horse serum and 1.7 nM insulin. The myogenic differentiation of this cell line was more rapid and obvious than that of C2C12 cells, as judged by morphological changes and expression of various muscle regulatory factors. To confirm that lack of the p53 and p16INK4a pathway does not prevent MyoD-mediated myogenesis, we established a cell line transformed with SV40LT (BEFV) and introduced MyoD into it. In the presence of 2% horse serum and 1.7 nM insulin, the MyoD-transduced BEFV cells differentiated like the MyoD-transduced BEFS cells, and displayed a similar pattern of expression of muscle regulatory proteins. Taken together, our results indicate that MyoD overexpression overcomes the defect in muscle differentiation associated with immortalization and cell transformation caused by the loss of p53 and Rb functions.

Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalMolecules and Cells
Volume21
Issue number2
Publication statusPublished - 2006 Apr 30

Fingerprint

Fibroblasts
Muscle Development
Muscles
Horses
Insulin
Cell Line
Transformed Cell Line
Desmin
Muscle Proteins
Serum
Cell Count

Keywords

  • BEF
  • Muscle differentiation
  • MyoD
  • p53
  • Rb
  • SV40LT

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Myogenic differentiation of p53- and Rb-deficient immortalized and transformed bovine fibroblasts in response to MyoD. / Jin, Xun; Lee, Joong Seub; Kwak, Sungwook; Jung, Ji Eun; Kim, Tae Kyung; Xu, Chenxiong; Hong, Zhongshan; Li, Zhehu; Kim, Sun Myoung; Whang, Kwang Youn; Hong, Ki Chang; You, Seungkwon; Choi, Yun Jaie; Kim, Hyunggee.

In: Molecules and Cells, Vol. 21, No. 2, 30.04.2006, p. 206-212.

Research output: Contribution to journalArticle

Jin, X, Lee, JS, Kwak, S, Jung, JE, Kim, TK, Xu, C, Hong, Z, Li, Z, Kim, SM, Whang, KY, Hong, KC, You, S, Choi, YJ & Kim, H 2006, 'Myogenic differentiation of p53- and Rb-deficient immortalized and transformed bovine fibroblasts in response to MyoD', Molecules and Cells, vol. 21, no. 2, pp. 206-212.
Jin, Xun ; Lee, Joong Seub ; Kwak, Sungwook ; Jung, Ji Eun ; Kim, Tae Kyung ; Xu, Chenxiong ; Hong, Zhongshan ; Li, Zhehu ; Kim, Sun Myoung ; Whang, Kwang Youn ; Hong, Ki Chang ; You, Seungkwon ; Choi, Yun Jaie ; Kim, Hyunggee. / Myogenic differentiation of p53- and Rb-deficient immortalized and transformed bovine fibroblasts in response to MyoD. In: Molecules and Cells. 2006 ; Vol. 21, No. 2. pp. 206-212.
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