Myogenic differentiation of p53- and Rb-deficient immortalized and transformed bovine fibroblasts in response to MyoD

Xun Jin, Joong Seub Lee, Sungwook Kwak, Ji Eun Jung, Tae Kyung Kim, Chenxiong Xu, Zhongshan Hong, Zhehu Li, Sun Myoung Kim, Kwang Youn Whang, Ki Chang Hong, Seungkwon You, Yun Jaie Choi, Hyunggee Kim

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14 Citations (Scopus)

Abstract

We have established in culture a spontaneously immortalized bovine embryonic fibroblast (BEF) cell line that has lost p53 and p16INK4a functions. MyoD is a muscle-specific regulator capable of inducing myogenesis in a number of cell types. When the BEF cells were transduced with MyoD they differentiated efficiently to desmin-positive myofibers in the presence of 2% horse serum and 1.7 nM insulin. The myogenic differentiation of this cell line was more rapid and obvious than that of C2C12 cells, as judged by morphological changes and expression of various muscle regulatory factors. To confirm that lack of the p53 and p16INK4a pathway does not prevent MyoD-mediated myogenesis, we established a cell line transformed with SV40LT (BEFV) and introduced MyoD into it. In the presence of 2% horse serum and 1.7 nM insulin, the MyoD-transduced BEFV cells differentiated like the MyoD-transduced BEFS cells, and displayed a similar pattern of expression of muscle regulatory proteins. Taken together, our results indicate that MyoD overexpression overcomes the defect in muscle differentiation associated with immortalization and cell transformation caused by the loss of p53 and Rb functions.

Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalMolecules and cells
Volume21
Issue number2
Publication statusPublished - 2006 Apr 30

Keywords

  • BEF
  • Muscle differentiation
  • MyoD
  • Rb
  • SV40LT
  • p53

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Jin, X., Lee, J. S., Kwak, S., Jung, J. E., Kim, T. K., Xu, C., Hong, Z., Li, Z., Kim, S. M., Whang, K. Y., Hong, K. C., You, S., Choi, Y. J., & Kim, H. (2006). Myogenic differentiation of p53- and Rb-deficient immortalized and transformed bovine fibroblasts in response to MyoD. Molecules and cells, 21(2), 206-212.