Myosin heavy chain composition of rat middle ear muscles

Hak Hyun Jung; Seung Hoon Han; Soon Yuhl Nam; Young Ho Kim; Jeung Lim Kim

doi:10.1080/00016480310002249

Myosin heavy chain composition of rat middle ear muscles

Hak Hyun Jung, Seung Hoon Han, Soon Yuhl Nam, Young Ho Kim, Jeung Lim Kim

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.

Original language	English
Pages (from-to)	569-573
Number of pages	5
Journal	Acta Oto-Laryngologica
Volume	124
Issue number	5
DOIs	https://doi.org/10.1080/00016480310002249
Publication status	Published - 2004
Externally published	Yes

Keywords

Branchial muscle
Muscle contraction
Rat

ASJC Scopus subject areas

Otorhinolaryngology

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10.1080/00016480310002249

Cite this

@article{e095c55ccdd341349c3a53857faa8080,

title = "Myosin heavy chain composition of rat middle ear muscles",

abstract = "Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.",

keywords = "Branchial muscle, Muscle contraction, Rat",

author = "Jung, {Hak Hyun} and Han, {Seung Hoon} and Nam, {Soon Yuhl} and Kim, {Young Ho} and Kim, {Jeung Lim}",

note = "Funding Information: This study was supported by the Brain Korea 21 Project of Korea University, and by a grant (00-178) from the Asan Institute for Life Sciences, Seoul, South Korea.",

year = "2004",

doi = "10.1080/00016480310002249",

language = "English",

volume = "124",

pages = "569--573",

journal = "Acta Oto-Laryngologica",

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number = "5",

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TY - JOUR

T1 - Myosin heavy chain composition of rat middle ear muscles

AU - Jung, Hak Hyun

AU - Han, Seung Hoon

AU - Nam, Soon Yuhl

AU - Kim, Young Ho

AU - Kim, Jeung Lim

N1 - Funding Information: This study was supported by the Brain Korea 21 Project of Korea University, and by a grant (00-178) from the Asan Institute for Life Sciences, Seoul, South Korea.

PY - 2004

Y1 - 2004

N2 - Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.

AB - Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.

KW - Branchial muscle

KW - Muscle contraction

KW - Rat

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DO - 10.1080/00016480310002249

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JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

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ER -

Myosin heavy chain composition of rat middle ear muscles

Abstract

Keywords

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