Abstract
Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.
| Original language | English |
|---|---|
| Pages (from-to) | 569-573 |
| Number of pages | 5 |
| Journal | Acta Oto-Laryngologica |
| Volume | 124 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2004 Jul 14 |
| Externally published | Yes |
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Keywords
- Branchial muscle
- Muscle contraction
- Rat
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Myosin heavy chain composition of rat middle ear muscles. / Jung, Hak Hyun; Han, Seung Hoon; Nam, Soon Yuhl; Kim, Young Ho; Kim, Jeung Lim.
In: Acta Oto-Laryngologica, Vol. 124, No. 5, 14.07.2004, p. 569-573.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Myosin heavy chain composition of rat middle ear muscles
AU - Jung, Hak Hyun
AU - Han, Seung Hoon
AU - Nam, Soon Yuhl
AU - Kim, Young Ho
AU - Kim, Jeung Lim
PY - 2004/7/14
Y1 - 2004/7/14
N2 - Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.
AB - Objective - To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods - An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results - The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion - These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant.
KW - Branchial muscle
KW - Muscle contraction
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=3042825494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042825494&partnerID=8YFLogxK
U2 - 10.1080/00016480310002249
DO - 10.1080/00016480310002249
M3 - Article
C2 - 15267173
AN - SCOPUS:3042825494
VL - 124
SP - 569
EP - 573
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
SN - 0001-6489
IS - 5
ER -