N-(4-hydroxyphenyl)retinamide inhibits breast cancer cell invasion through suppressing NF-KB activation and inhibiting matrix metalloproteinase-9 expression

Hyereen Kang, Minjae Lee, Kyung Chul Choi, Dong Myoung Shin, Je Sang Ko, Sung Wuk Jang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Synthetic retinoid N-(4-hydroxyphenyl)retinamide (4-HPR) has been reported to exhibit anti-invasive and anti-metastatic activities by suppressing the enzymatic activity of matrix metalloproteinase (MMP)-9, but the underlying mechanism remains unclear. Here, we show that 4-HPR blocks the activity of MMP-9 in two ways: by reducing phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 secretion and by suppressing cell invasion through the downregulation of MMP-9 gene transcription in MCF-7 breast cancer cells. 4-HPR inhibits the transcriptional activity of MMP-9 by reducing the DNA-binding activity of NF-κB on the MMP-9 promoter as well as by inhibiting the degradation of IκBα, leading to cytoplasmic accumulation of NF-κB. We also found that 4-HPR inhibits invasion and MMP-9 expression in the highly metastatic breast cancer cell line MDA-MB-231. Thus, 4-HPR might be a potent anti-invasive agent that works by suppressing MMP-9 expression via the NF-κB signaling pathway.

Original languageEnglish
Pages (from-to)2845-2855
Number of pages11
JournalJournal of Cellular Biochemistry
Volume113
Issue number9
DOIs
Publication statusPublished - 2012 Sep 1

Fingerprint

Fenretinide
Matrix Metalloproteinase 9
Chemical activation
Cells
Breast Neoplasms
Retinoids
Transcription
Acetates
Down-Regulation
Genes
Cell Line
Degradation

Keywords

  • 4-HPR
  • breast cancer
  • cell invasion
  • MMP-9
  • NF-κB

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

N-(4-hydroxyphenyl)retinamide inhibits breast cancer cell invasion through suppressing NF-KB activation and inhibiting matrix metalloproteinase-9 expression. / Kang, Hyereen; Lee, Minjae; Choi, Kyung Chul; Shin, Dong Myoung; Ko, Je Sang; Jang, Sung Wuk.

In: Journal of Cellular Biochemistry, Vol. 113, No. 9, 01.09.2012, p. 2845-2855.

Research output: Contribution to journalArticle

@article{4421fb621b894e44aa52f67d7ac1f4af,
title = "N-(4-hydroxyphenyl)retinamide inhibits breast cancer cell invasion through suppressing NF-KB activation and inhibiting matrix metalloproteinase-9 expression",
abstract = "Synthetic retinoid N-(4-hydroxyphenyl)retinamide (4-HPR) has been reported to exhibit anti-invasive and anti-metastatic activities by suppressing the enzymatic activity of matrix metalloproteinase (MMP)-9, but the underlying mechanism remains unclear. Here, we show that 4-HPR blocks the activity of MMP-9 in two ways: by reducing phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 secretion and by suppressing cell invasion through the downregulation of MMP-9 gene transcription in MCF-7 breast cancer cells. 4-HPR inhibits the transcriptional activity of MMP-9 by reducing the DNA-binding activity of NF-κB on the MMP-9 promoter as well as by inhibiting the degradation of IκBα, leading to cytoplasmic accumulation of NF-κB. We also found that 4-HPR inhibits invasion and MMP-9 expression in the highly metastatic breast cancer cell line MDA-MB-231. Thus, 4-HPR might be a potent anti-invasive agent that works by suppressing MMP-9 expression via the NF-κB signaling pathway.",
keywords = "4-HPR, breast cancer, cell invasion, MMP-9, NF-κB",
author = "Hyereen Kang and Minjae Lee and Choi, {Kyung Chul} and Shin, {Dong Myoung} and Ko, {Je Sang} and Jang, {Sung Wuk}",
year = "2012",
month = "9",
day = "1",
doi = "10.1002/jcb.24159",
language = "English",
volume = "113",
pages = "2845--2855",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "9",

}

TY - JOUR

T1 - N-(4-hydroxyphenyl)retinamide inhibits breast cancer cell invasion through suppressing NF-KB activation and inhibiting matrix metalloproteinase-9 expression

AU - Kang, Hyereen

AU - Lee, Minjae

AU - Choi, Kyung Chul

AU - Shin, Dong Myoung

AU - Ko, Je Sang

AU - Jang, Sung Wuk

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Synthetic retinoid N-(4-hydroxyphenyl)retinamide (4-HPR) has been reported to exhibit anti-invasive and anti-metastatic activities by suppressing the enzymatic activity of matrix metalloproteinase (MMP)-9, but the underlying mechanism remains unclear. Here, we show that 4-HPR blocks the activity of MMP-9 in two ways: by reducing phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 secretion and by suppressing cell invasion through the downregulation of MMP-9 gene transcription in MCF-7 breast cancer cells. 4-HPR inhibits the transcriptional activity of MMP-9 by reducing the DNA-binding activity of NF-κB on the MMP-9 promoter as well as by inhibiting the degradation of IκBα, leading to cytoplasmic accumulation of NF-κB. We also found that 4-HPR inhibits invasion and MMP-9 expression in the highly metastatic breast cancer cell line MDA-MB-231. Thus, 4-HPR might be a potent anti-invasive agent that works by suppressing MMP-9 expression via the NF-κB signaling pathway.

AB - Synthetic retinoid N-(4-hydroxyphenyl)retinamide (4-HPR) has been reported to exhibit anti-invasive and anti-metastatic activities by suppressing the enzymatic activity of matrix metalloproteinase (MMP)-9, but the underlying mechanism remains unclear. Here, we show that 4-HPR blocks the activity of MMP-9 in two ways: by reducing phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 secretion and by suppressing cell invasion through the downregulation of MMP-9 gene transcription in MCF-7 breast cancer cells. 4-HPR inhibits the transcriptional activity of MMP-9 by reducing the DNA-binding activity of NF-κB on the MMP-9 promoter as well as by inhibiting the degradation of IκBα, leading to cytoplasmic accumulation of NF-κB. We also found that 4-HPR inhibits invasion and MMP-9 expression in the highly metastatic breast cancer cell line MDA-MB-231. Thus, 4-HPR might be a potent anti-invasive agent that works by suppressing MMP-9 expression via the NF-κB signaling pathway.

KW - 4-HPR

KW - breast cancer

KW - cell invasion

KW - MMP-9

KW - NF-κB

UR - http://www.scopus.com/inward/record.url?scp=84863643540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863643540&partnerID=8YFLogxK

U2 - 10.1002/jcb.24159

DO - 10.1002/jcb.24159

M3 - Article

C2 - 22488409

AN - SCOPUS:84863643540

VL - 113

SP - 2845

EP - 2855

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 9

ER -