N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Y. H. Noh, H. S. Cho, D. H. Kim, O. H. Kim, J. Park, S. A. Lee, H. S. Yang, D. S. Sohn, W. Kim, D. Kim, Y. H. Chung, K. Y. Kim, S. S. Kim, W. B. Lee

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2 Citations (Scopus)

Abstract

We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

Original languageEnglish
Pages (from-to)664-669
Number of pages6
JournalMolecular Biology
Volume46
Issue number5
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Akt
  • N-acetylcysteine
  • N-cadherin
  • P19 embryonic stem cell
  • differentiation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

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  • Cite this

    Noh, Y. H., Cho, H. S., Kim, D. H., Kim, O. H., Park, J., Lee, S. A., Yang, H. S., Sohn, D. S., Kim, W., Kim, D., Chung, Y. H., Kim, K. Y., Kim, S. S., & Lee, W. B. (2012). N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation. Molecular Biology, 46(5), 664-669. https://doi.org/10.1134/S0026893312040085