N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Y. H. Noh, H. S. Cho, D. H. Kim, O. H. Kim, J. Park, S. A. Lee, H. S. Yang, D. S. Sohn, Won-Ki Kim, D. Kim, Y. H. Chung, K. Y. Kim, S. S. Kim, W. B. Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

Original languageEnglish
Pages (from-to)664-669
Number of pages6
JournalMolecular Biology
Volume46
Issue number5
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Acetylcysteine
Cadherins
Embryonic Stem Cells
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Microtubule-Associated Proteins
Protein-Serine-Threonine Kinases
Neurons

Keywords

  • Akt
  • differentiation
  • N-acetylcysteine
  • N-cadherin
  • P19 embryonic stem cell

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics

Cite this

N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation. / Noh, Y. H.; Cho, H. S.; Kim, D. H.; Kim, O. H.; Park, J.; Lee, S. A.; Yang, H. S.; Sohn, D. S.; Kim, Won-Ki; Kim, D.; Chung, Y. H.; Kim, K. Y.; Kim, S. S.; Lee, W. B.

In: Molecular Biology, Vol. 46, No. 5, 2012, p. 664-669.

Research output: Contribution to journalArticle

Noh, YH, Cho, HS, Kim, DH, Kim, OH, Park, J, Lee, SA, Yang, HS, Sohn, DS, Kim, W-K, Kim, D, Chung, YH, Kim, KY, Kim, SS & Lee, WB 2012, 'N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation', Molecular Biology, vol. 46, no. 5, pp. 664-669. https://doi.org/10.1134/S0026893312040085
Noh, Y. H. ; Cho, H. S. ; Kim, D. H. ; Kim, O. H. ; Park, J. ; Lee, S. A. ; Yang, H. S. ; Sohn, D. S. ; Kim, Won-Ki ; Kim, D. ; Chung, Y. H. ; Kim, K. Y. ; Kim, S. S. ; Lee, W. B. / N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation. In: Molecular Biology. 2012 ; Vol. 46, No. 5. pp. 664-669.
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AB - We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

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