N-terminal Processing Is Essential for Release of Epithin, a Mouse Type II Membrane Serine Protease

Eun Gyung Cho, Moon Gyo Kim, Chungho Kim, Seung Ryul Kim, Ihn Sik Seong, Chinha Chung, Ronald H. Schwartz, Dongeun Park

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70 Citations (Scopus)

Abstract

Epithin was originally identified as a mouse type II membrane serine protease. Its human orthologue membrane type-serine protease 1 (MT-SP1)/matriptase has been reported to be localized on the plasma membrane. In addition, soluble forms of matriptase were isolated from human breast milk and breast cancer cell-conditioned medium. In this paper, we report a processing mechanism that appears to be required for the release of epithin. CHO-K1 or COS7 cells transfected with single full-length epithin cDNA generated two different-sized proteins in cell lysates, 110 and 92 kDa. The 92-kDa epithin was found to be an N-terminally truncated form of the 110-kDa epithin, and it was the only form detected in the culture medium. The 92-kDa epithin was also found on the cell surface, where it was anchored by the N-terminal fragment. The results of in vivo cell labeling experiments indicate that the 110-kDa epithin is rapidly processed to the 92-kDa epithin. Using site-directed mutagenesis experiments, we identified Gly149 of the GSVIA sequence in epithin as required for the processing and release of the protein. These results suggest that N-terminal proeessing processing of epithin at Gly 149 is a necessary prerequisite step for release of the protein.

Original languageEnglish
Pages (from-to)44581-44589
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number48
DOIs
Publication statusPublished - 2001 Nov 30

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Cho, E. G., Kim, M. G., Kim, C., Kim, S. R., Seong, I. S., Chung, C., Schwartz, R. H., & Park, D. (2001). N-terminal Processing Is Essential for Release of Epithin, a Mouse Type II Membrane Serine Protease. Journal of Biological Chemistry, 276(48), 44581-44589. https://doi.org/10.1074/jbc.M107059200