Nanobiocatalysis for protein digestion in proteomic analysis

Jungbae Kim, Byoung Chan Kim, Daniel Lopez-Ferrer, Konstantinos Petritis, Richard D. Smith

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The process of protein digestion is a critical step for successful protein identification in bottom-up proteomic analyses. To substitute the present practice of in-solution protein digestion, which is long, tedious, and difficult to automate, many efforts have been dedicated for the development of a rapid, recyclable and automated digestion system. Recent advances of nanobiocatalytic approaches have improved the performance of protein digestion by using various nanomaterials such as nanoporous materials, magnetic nanoparticles, and polymer nanofibers. Especially, the unprecedented success of trypsin stabilization in the form of trypsin-coated nanofibers, showing no activity decrease under repeated uses for 1 year and retaining good resistance to proteolysis, has demonstrated its great potential to be employed in the development of automated, high-throughput, and on-line digestion systems. This review discusses recent developments of nanobiocatalytic approaches for the improved performance of protein digestion in speed, detection sensitivity, recyclability, and trypsin stability. In addition, we also introduce approaches for protein digestion under unconventional energy input for protein denaturation and the development of microfluidic enzyme reactors that can benefit from recent successes of these nanobiocatalytic approaches. & 2010 WILEY-VCH Verlag GmbH & Co. KGaA.

Original languageEnglish
Pages (from-to)687-699
Number of pages13
JournalProteomics
Volume10
Issue number4
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Proteomics
Proteolysis
Trypsin
Nanofibers
Proteins
Digestion
Online Systems
Protein Denaturation
Microfluidics
Nanostructures
Denaturation
Magnetic materials
Nanoparticles
Nanostructured materials
Polymers
Stabilization
Throughput
Enzymes

Keywords

  • Enzyme coating
  • Nanobiocatalysis
  • Nanoproteomics
  • Protein digestion
  • Trypsin stabilization

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Kim, J., Kim, B. C., Lopez-Ferrer, D., Petritis, K., & Smith, R. D. (2010). Nanobiocatalysis for protein digestion in proteomic analysis. Proteomics, 10(4), 687-699. https://doi.org/10.1002/pmic.200900519

Nanobiocatalysis for protein digestion in proteomic analysis. / Kim, Jungbae; Kim, Byoung Chan; Lopez-Ferrer, Daniel; Petritis, Konstantinos; Smith, Richard D.

In: Proteomics, Vol. 10, No. 4, 01.02.2010, p. 687-699.

Research output: Contribution to journalArticle

Kim, J, Kim, BC, Lopez-Ferrer, D, Petritis, K & Smith, RD 2010, 'Nanobiocatalysis for protein digestion in proteomic analysis', Proteomics, vol. 10, no. 4, pp. 687-699. https://doi.org/10.1002/pmic.200900519
Kim J, Kim BC, Lopez-Ferrer D, Petritis K, Smith RD. Nanobiocatalysis for protein digestion in proteomic analysis. Proteomics. 2010 Feb 1;10(4):687-699. https://doi.org/10.1002/pmic.200900519
Kim, Jungbae ; Kim, Byoung Chan ; Lopez-Ferrer, Daniel ; Petritis, Konstantinos ; Smith, Richard D. / Nanobiocatalysis for protein digestion in proteomic analysis. In: Proteomics. 2010 ; Vol. 10, No. 4. pp. 687-699.
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