Nanoelectrokinetic-assisted lateral flow assay for COVID-19 antibody test

Cheonjung Kim, Yong Kyoung Yoo, Na Eun Lee, Junwoo Lee, Kang Hyeon Kim, Seungmin Lee, Jinhwan Kim, Seong Jun Park, Dongtak Lee, Sang Won Lee, Kyo Seon Hwang, Sung Il Han, Dongho Lee, Dae Sung Yoon, Jeong Hoon Lee

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A lateral flow assay (LFA) platform is a powerful tool for point-of-care testing (POCT), especially for self-testing. Although the LFA platform provides a simple and disposable tool for Coronavirus disease of 2019 (COVID-19) antigen (Ag) and antibody (Ab) screening tests, the lower sensitivity for low virus titers has been a bottleneck for practical applications. Herein, we report the combination of a microfluidic paper-based nanoelectrokinetic (NEK) preconcentrator and an LFA platform for enhancing the sensitivity and limit of detection (LOD). Biomarkers were electrokinetically preconcentrated onto a specific layer using the NEK preconcentrator, which was then coupled with LFA diagnostic devices for enhanced performance. Using this nanoelectrokinetic-assisted LFA (NEK-LFA) platform for self-testing, the severe acute respiratory syndrome coronavirus 2 Immunoglobulin G (SARS-CoV-2 IgG) sample was preconcentrated from serum samples. After preconcentration, the LOD of the LFA was enhanced by 32-fold, with an increase in analytical sensitivity (16.4%), which may offer a new opportunity for POCT and self-testing, especially in the COVID-19 pandemic and endemic global context.

Original languageEnglish
Article number114385
JournalBiosensors and Bioelectronics
Volume212
DOIs
Publication statusPublished - 2022 Sep 15

Keywords

  • Biomarker
  • COVID-19
  • Lateral flow assay
  • Preconcentration
  • SARS-CoV-2 IgG
  • Self-testing

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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