Nanografted Substrata and Triculture of Human Pericytes, Fibroblasts, and Endothelial Cells for Studying the Effects on Angiogenesis

Tae Hee Kim, Soo Hyun Kim, Kam W. Leong, Youngmee Jung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

For the successful treatment of severe wounds, angiogenesis must be induced to provide a sufficient blood supply to the wound site. There have been many studies on various structural and biochemical factors regulating angiogenesis, such as surface topography, surface modifications, angiogenic factors, and various cell types. However, there is a paucity of research on the effects of micro- and nanoscale topography and the application of pericytes in angiogenesis. In this study, we utilized nanoscale topography combined with a triculture system consisting of human pericytes, fibroblasts, and endothelial cells. We investigated the effects of the nanografted substrata and a triculture system on proangiogenic therapies in vitro. Human dermal fibroblasts and human umbilical vein endothelial cells were seeded with human pericytes from the placenta directly onto nanografted substrata composed of polydimethylsiloxane. We demonstrated that key elements of angiogenesis, including segment and capillary-like tubular structure formation, as well as the secretion of extracellular matrix, were increased by interaction between the nanografted substrata and the coculture containing pericytes. Thus, nanografted surfaces and triculture systems containing human pericytes, fibroblasts, and endothelial cells promote angiogenesis.

Original languageEnglish
Pages (from-to)698-706
Number of pages9
JournalTissue Engineering - Part A
Volume22
Issue number7-8
DOIs
Publication statusPublished - 2016 Apr 1

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Pericytes
Endothelial cells
Fibroblasts
Endothelial Cells
Angiogenesis Inducing Agents
Topography
Human Umbilical Vein Endothelial Cells
Wounds and Injuries
Surface topography
Polydimethylsiloxane
Coculture Techniques
Placenta
Extracellular Matrix
Surface treatment
Blood
Skin
Research

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Nanografted Substrata and Triculture of Human Pericytes, Fibroblasts, and Endothelial Cells for Studying the Effects on Angiogenesis. / Kim, Tae Hee; Kim, Soo Hyun; Leong, Kam W.; Jung, Youngmee.

In: Tissue Engineering - Part A, Vol. 22, No. 7-8, 01.04.2016, p. 698-706.

Research output: Contribution to journalArticle

Kim, Tae Hee ; Kim, Soo Hyun ; Leong, Kam W. ; Jung, Youngmee. / Nanografted Substrata and Triculture of Human Pericytes, Fibroblasts, and Endothelial Cells for Studying the Effects on Angiogenesis. In: Tissue Engineering - Part A. 2016 ; Vol. 22, No. 7-8. pp. 698-706.
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