Nanoimmobilization of marine epoxide hydrolase of mugil cephalus for repetitive enantioselective resolution of racemic styrene oxide in aqueous buffer

Young Hyun Kim, Inseon Lee, Sung Hee Choi, Ok Kyung Lee, Jungbae Kim, Eun Yeol Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We developed two nanoimmobilized biocatalyst systems of thermally unstable Mugil cephalus epoxide hydrolase (McEH) for enantioselective resolution of racemic styrene oxide in aqueous buffer. The recombinant and purified McEH enzyme was immobilized onto magnetic nanoparticles (Mag-NPs) via a two step process of enzyme precipitation and crosslinking. McEH enzyme was also adsorbed, precipitated, and cross-linked in/on polyaniline nanofibers (PANFs). The residual relative activity of free McEH, defined as the ratio of residual activity to the initial activity, was 8% after incubation at 30 °C for 80 h while those of McEH immobilized onto Mag-NPs and in/on PANFs were 15% and 33% in the same condition, respectively. McEH immobilizations onto Mag-NPs and in/on PANFs could be reused in seven repetitive batch reactions for enantioselective hydrolysis of racemic styrene oxide to prepare (S)-styrene oxide with 98% enantiomeric excess (ee) while retaining greater than 40-50% of their initial activity.

Original languageEnglish
Pages (from-to)2266-2271
Number of pages6
JournalJournal of Nanoscience and Nanotechnology
Volume13
Issue number3
DOIs
Publication statusPublished - 2013 Mar

Keywords

  • Enantioselective resolution
  • Epoxide hydrolase
  • Magnetic nanoparticle
  • Nanoimmobilization
  • Polyaniline nanofiber

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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