Nanoliposomal Ratiometric Fluorescent Probe toward ONOO-Flux

Jie Zhang, Jianfei Kan, Yanyan Sun, Miae Won, Ji Hyeon Kim, Weifen Zhang, Jin Zhou, Zhaosheng Qian, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxynitrite (ONOO-), a powerful biological oxidant, is produced in the mitochondria and reacts with many biomolecular targets under various pathological conditions, leading to a range of disease states. In this work, we developed a nanoliposome-encapsulated ratiometrically fluorescent probe (NRF) based on a hemicyanine structure Cy-O obtained by facile synthesis. Upon reaction with ONOO-, the oxidation and hydrolysis of a π-conjugation system within the nanoliposome triggers a ratiometrically fluorescent response and a large-scale emission shift (238 nm), which provides a specific and sensitive means for the ONOO- detection. Moreover, we have performed DFT calculation at the 6-31+G(d,p) level using a suite of Gaussian 09 programs to obtain insights into the chemical structure optical properties of Cy-O. In addition, the practical applications of the nanoprobe to image exogenous and endogenous ONOO- were achieved further in live cells and animals triumphantly.

Original languageEnglish
Pages (from-to)2080-2088
Number of pages9
JournalACS Applied Bio Materials
Volume4
Issue number3
DOIs
Publication statusPublished - 2021 Mar 15

Keywords

  • fluorescent probe
  • imaging
  • nanoliposome
  • peroxynitrite
  • ratiometric

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, medical

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