Nanomechanical measurement of astrocyte stiffness correlated with cytoskeletal maturation

Sang Myung Lee, Thi Huong Nguyen, Kyounghwan Na, Il Joo Cho, Dong Ho Woo, Jae Eung Oh, Changjoon Lee, Eui Sung Yoon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Astrocytes are known to serve as scaffolding cells that shape the brain. The physical properties of astrocytes, such as stiffness, are important for their scaffolding function. These properties may be altered in certain pathological conditions, such as in brain cancer. However, actual stiffness of astrocytes is not yet well understood. Here, we report that the astrocyte stiffness is positively correlated with the density of cytoskeletal proteins, such as actin filaments, microtubules, and intermediate filaments. The value of the stiffness of astrocytes as measured by atomic force microscopy (AFM) increases 38-fold in five-week-old rats compared to postnatal-day zero pups. Using multicolor confocal microscopy, we found that the complexity of cytoskeletal proteins, such as actin filaments, microtubules, and intermediate filaments, increase as the animal gets older. Our findings indicate that the change of stiffness positively correlates with the maturation of cytoskeletal proteins, and suggest that AFM can be useful as an analytical and diagnostic tool for neuroscience.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Stiffness
Cytoskeletal Proteins
Proteins
Actins
Atomic force microscopy
Brain
Confocal microscopy
Rats
Animals
Physical properties
Astrocytes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Nanomechanical measurement of astrocyte stiffness correlated with cytoskeletal maturation. / Lee, Sang Myung; Nguyen, Thi Huong; Na, Kyounghwan; Cho, Il Joo; Woo, Dong Ho; Oh, Jae Eung; Lee, Changjoon; Yoon, Eui Sung.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 1, 01.01.2014, p. 365-370.

Research output: Contribution to journalArticle

Lee, Sang Myung ; Nguyen, Thi Huong ; Na, Kyounghwan ; Cho, Il Joo ; Woo, Dong Ho ; Oh, Jae Eung ; Lee, Changjoon ; Yoon, Eui Sung. / Nanomechanical measurement of astrocyte stiffness correlated with cytoskeletal maturation. In: Journal of Biomedical Materials Research - Part A. 2014 ; Vol. 103, No. 1. pp. 365-370.
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