Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase

Kyo Seon Hwang, Sang Myung Lee, Kilho Eom, Jeong Hoon Lee, Yoon Sik Lee, Jung ho Park, Dae Sung Yoon, Tae Song Kim

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalBiosensors and Bioelectronics
Volume23
Issue number4
DOIs
Publication statusPublished - 2007 Nov 30

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ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

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