Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions

Donghee Son, Sung Young Park, Byeongju Kim, Jun Tae Koh, Tae Hyun Kim, Sangmin An, Doyoung Jang, Gyu Tae Kim, Wonho Jhe, Seunghun Hong

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We developed a nanoneedle transistor-based sensor (NTS) for the selective detection of calcium ions inside a living cell. In this work, a single-walled carbon nanotube-based field effect transistor (swCNT-FET) was first fabricated at the end of a glass nanopipette and functionalized with Fluo-4-AM probe dye. The selective binding of calcium ions onto the dye molecules altered the charge state of the dye molecules, resulting in the change of the source-drain current of the swCNT-FET as well as the fluorescence intensity from the dye. We demonstrated the electrical and fluorescence detection of the concentration change of intracellular calcium ions inside a HeLa cell using the NTS.

Original languageEnglish
Pages (from-to)3888-3895
Number of pages8
JournalACS nano
Volume5
Issue number5
DOIs
Publication statusPublished - 2011 May 24

Keywords

  • Carbon nanotube
  • Field effect transistor
  • Fluo-4-AM dye
  • Intracellular calcium ion
  • Nanoneedle transistor-based sensor
  • Real-time sensor

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions'. Together they form a unique fingerprint.

  • Cite this

    Son, D., Park, S. Y., Kim, B., Koh, J. T., Kim, T. H., An, S., Jang, D., Kim, G. T., Jhe, W., & Hong, S. (2011). Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions. ACS nano, 5(5), 3888-3895. https://doi.org/10.1021/nn200262u