Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions

Donghee Son, Sung Young Park, Byeongju Kim, Jun Tae Koh, Tae Hyun Kim, Sangmin An, Doyoung Jang, Gyu-Tae Kim, Wonho Jhe, Seunghun Hong

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We developed a nanoneedle transistor-based sensor (NTS) for the selective detection of calcium ions inside a living cell. In this work, a single-walled carbon nanotube-based field effect transistor (swCNT-FET) was first fabricated at the end of a glass nanopipette and functionalized with Fluo-4-AM probe dye. The selective binding of calcium ions onto the dye molecules altered the charge state of the dye molecules, resulting in the change of the source-drain current of the swCNT-FET as well as the fluorescence intensity from the dye. We demonstrated the electrical and fluorescence detection of the concentration change of intracellular calcium ions inside a HeLa cell using the NTS.

Original languageEnglish
Pages (from-to)3888-3895
Number of pages8
JournalACS Nano
Volume5
Issue number5
DOIs
Publication statusPublished - 2011 May 24

Fingerprint

Nanoneedles
calcium
Calcium
Transistors
Coloring Agents
transistors
Dyes
dyes
Ions
sensors
Sensors
Single-walled carbon nanotubes (SWCN)
Field effect transistors
ions
field effect transistors
Fluorescence
carbon nanotubes
fluorescence
Molecules
Drain current

Keywords

  • Carbon nanotube
  • Field effect transistor
  • Fluo-4-AM dye
  • Intracellular calcium ion
  • Nanoneedle transistor-based sensor
  • Real-time sensor

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Son, D., Park, S. Y., Kim, B., Koh, J. T., Kim, T. H., An, S., ... Hong, S. (2011). Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions. ACS Nano, 5(5), 3888-3895. https://doi.org/10.1021/nn200262u

Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions. / Son, Donghee; Park, Sung Young; Kim, Byeongju; Koh, Jun Tae; Kim, Tae Hyun; An, Sangmin; Jang, Doyoung; Kim, Gyu-Tae; Jhe, Wonho; Hong, Seunghun.

In: ACS Nano, Vol. 5, No. 5, 24.05.2011, p. 3888-3895.

Research output: Contribution to journalArticle

Son, D, Park, SY, Kim, B, Koh, JT, Kim, TH, An, S, Jang, D, Kim, G-T, Jhe, W & Hong, S 2011, 'Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions', ACS Nano, vol. 5, no. 5, pp. 3888-3895. https://doi.org/10.1021/nn200262u
Son, Donghee ; Park, Sung Young ; Kim, Byeongju ; Koh, Jun Tae ; Kim, Tae Hyun ; An, Sangmin ; Jang, Doyoung ; Kim, Gyu-Tae ; Jhe, Wonho ; Hong, Seunghun. / Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions. In: ACS Nano. 2011 ; Vol. 5, No. 5. pp. 3888-3895.
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