Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions

Donghee Son, Sung Young Park, Byeongju Kim, Jun Tae Koh, Tae Hyun Kim, Sangmin An, Doyoung Jang, Gyu-Tae Kim, Wonho Jhe, Seunghun Hong

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We developed a nanoneedle transistor-based sensor (NTS) for the selective detection of calcium ions inside a living cell. In this work, a single-walled carbon nanotube-based field effect transistor (swCNT-FET) was first fabricated at the end of a glass nanopipette and functionalized with Fluo-4-AM probe dye. The selective binding of calcium ions onto the dye molecules altered the charge state of the dye molecules, resulting in the change of the source-drain current of the swCNT-FET as well as the fluorescence intensity from the dye. We demonstrated the electrical and fluorescence detection of the concentration change of intracellular calcium ions inside a HeLa cell using the NTS.

Original languageEnglish
Pages (from-to)3888-3895
Number of pages8
JournalACS Nano
Volume5
Issue number5
DOIs
Publication statusPublished - 2011 May 24

Keywords

  • Carbon nanotube
  • Field effect transistor
  • Fluo-4-AM dye
  • Intracellular calcium ion
  • Nanoneedle transistor-based sensor
  • Real-time sensor

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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  • Cite this

    Son, D., Park, S. Y., Kim, B., Koh, J. T., Kim, T. H., An, S., Jang, D., Kim, G-T., Jhe, W., & Hong, S. (2011). Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions. ACS Nano, 5(5), 3888-3895. https://doi.org/10.1021/nn200262u