Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

Ha Rim Seo, Hyung Joon Joo, Dae Hwan Kim, Long Hui Cui, Seung Cheol Choi, Jong Ho Kim, Sung Woo Cho, Kyu Back Lee, Do-Sun Lim

Research output: Contribution to journalArticle

8 Citations (Scopus)


Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.

Original languageEnglish
Pages (from-to)16803-16812
Number of pages10
JournalACS Applied Materials and Interfaces
Issue number20
Publication statusPublished - 2017 May 24



  • cardiomyocyte differentiation
  • cytoskeleton reorganization
  • Flk1-positive mesodermal precursor cells
  • gradient nanopattern plates
  • nanoimprinting

ASJC Scopus subject areas

  • Materials Science(all)

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