Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

Ha Rim Seo, Hyung Joon Joo, Dae Hwan Kim, Long Hui Cui, Seung Cheol Choi, Jong Ho Kim, Sung Woo Cho, Kyu Back Lee, Do-Sun Lim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.

Original languageEnglish
Pages (from-to)16803-16812
Number of pages10
JournalACS Applied Materials and Interfaces
Volume9
Issue number20
DOIs
Publication statusPublished - 2017 May 24

Fingerprint

Actin Depolymerizing Factors
Topology
Stem cells
Vinculin
Vascular Endothelial Growth Factor Receptor-2
Cell proliferation
Cell culture
Gene expression
Liver
Genes

Keywords

  • cardiomyocyte differentiation
  • cytoskeleton reorganization
  • Flk1-positive mesodermal precursor cells
  • gradient nanopattern plates
  • nanoimprinting

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement. / Seo, Ha Rim; Joo, Hyung Joon; Kim, Dae Hwan; Cui, Long Hui; Choi, Seung Cheol; Kim, Jong Ho; Cho, Sung Woo; Lee, Kyu Back; Lim, Do-Sun.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 20, 24.05.2017, p. 16803-16812.

Research output: Contribution to journalArticle

Seo, Ha Rim ; Joo, Hyung Joon ; Kim, Dae Hwan ; Cui, Long Hui ; Choi, Seung Cheol ; Kim, Jong Ho ; Cho, Sung Woo ; Lee, Kyu Back ; Lim, Do-Sun. / Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement. In: ACS Applied Materials and Interfaces. 2017 ; Vol. 9, No. 20. pp. 16803-16812.
@article{d8dc57023a8447fdbd3f8a6a73ded3df,
title = "Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement",
abstract = "Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.",
keywords = "cardiomyocyte differentiation, cytoskeleton reorganization, Flk1-positive mesodermal precursor cells, gradient nanopattern plates, nanoimprinting",
author = "Seo, {Ha Rim} and Joo, {Hyung Joon} and Kim, {Dae Hwan} and Cui, {Long Hui} and Choi, {Seung Cheol} and Kim, {Jong Ho} and Cho, {Sung Woo} and Lee, {Kyu Back} and Do-Sun Lim",
year = "2017",
month = "5",
day = "24",
doi = "10.1021/acsami.7b01555",
language = "English",
volume = "9",
pages = "16803--16812",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "20",

}

TY - JOUR

T1 - Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

AU - Seo, Ha Rim

AU - Joo, Hyung Joon

AU - Kim, Dae Hwan

AU - Cui, Long Hui

AU - Choi, Seung Cheol

AU - Kim, Jong Ho

AU - Cho, Sung Woo

AU - Lee, Kyu Back

AU - Lim, Do-Sun

PY - 2017/5/24

Y1 - 2017/5/24

N2 - Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.

AB - Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.

KW - cardiomyocyte differentiation

KW - cytoskeleton reorganization

KW - Flk1-positive mesodermal precursor cells

KW - gradient nanopattern plates

KW - nanoimprinting

UR - http://www.scopus.com/inward/record.url?scp=85019685957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019685957&partnerID=8YFLogxK

U2 - 10.1021/acsami.7b01555

DO - 10.1021/acsami.7b01555

M3 - Article

VL - 9

SP - 16803

EP - 16812

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 20

ER -