Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

Ha Rim Seo, Hyung Joon Joo, Dae Hwan Kim, Long Hui Cui, Seung Cheol Choi, Jong Ho Kim, Sung Woo Cho, Kyu Back Lee, Do-Sun Lim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.

Original languageEnglish
Pages (from-to)16803-16812
Number of pages10
JournalACS Applied Materials and Interfaces
Volume9
Issue number20
DOIs
Publication statusPublished - 2017 May 24

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Keywords

  • cardiomyocyte differentiation
  • cytoskeleton reorganization
  • Flk1-positive mesodermal precursor cells
  • gradient nanopattern plates
  • nanoimprinting

ASJC Scopus subject areas

  • Materials Science(all)

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