Nanoscale characterization of Escherichia coli biofilm formed under laminar flow using atomic force microscopy (AFM) and scanning electron microscopy (SEM)

Jeesun Lim, Kang Mu Lee, Hyun Kim So, Seong Won Nam, Jin Oh Yoo, Sun Yun Hyun, William Jo, Sejong Oh, Sae Hun Kim, Sungsu Park

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Biofilm contains heterogeneous three-dimensional structures composed of extracellular polymeric substance (EPS), which are greatly influenced by flow conditions. Here, we report a microfluidic platform highly suitable for nanoscale investigation of biofilms formed under laminar flows. This is possible because biofilms formed on glass beads having an average diameter of about 200 μm in the microfluidic device can be easily taken out and located for imaging under high resolution microscopes, such as atomic force microscope (AFM) and scanning electron microscope (SEM). Escherichia coli formed biofilms in the device at various flow conditions (0-50 μL min-1) for several days. SEM showed nanopores (14-100 nm) in biofilm at lower flow rates (0.5 and 5 μL min-1) only at within 3 days, while such small pores were not observed at higher flow rates (50 μL min-1) during the entire culture period (0-5 days). AFM results showed that the surface coverage and roughness of biofilm increased as the flow rate increased. These results suggest that like turbulent flow regime flow rates under laminar flow regime greatly influence on the morphology of biofilms in both micro- and nanoscales.

Original languageEnglish
Pages (from-to)2114-2118
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume29
Issue number11
Publication statusPublished - 2008 Nov 20

Fingerprint

Biofilms
Laminar flow
Escherichia coli
Atomic force microscopy
Scanning electron microscopy
Flow rate
Microfluidics
Microscopes
Nanopores
Turbulent flow
Electron microscopes
Surface roughness
Scanning
Imaging techniques
Glass

Keywords

  • AFM
  • Biofilm
  • Escherichia coli O157:H7
  • Nanostructure
  • SEM

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Nanoscale characterization of Escherichia coli biofilm formed under laminar flow using atomic force microscopy (AFM) and scanning electron microscopy (SEM). / Lim, Jeesun; Lee, Kang Mu; So, Hyun Kim; Nam, Seong Won; Yoo, Jin Oh; Hyun, Sun Yun; Jo, William; Oh, Sejong; Kim, Sae Hun; Park, Sungsu.

In: Bulletin of the Korean Chemical Society, Vol. 29, No. 11, 20.11.2008, p. 2114-2118.

Research output: Contribution to journalArticle

Lim, Jeesun ; Lee, Kang Mu ; So, Hyun Kim ; Nam, Seong Won ; Yoo, Jin Oh ; Hyun, Sun Yun ; Jo, William ; Oh, Sejong ; Kim, Sae Hun ; Park, Sungsu. / Nanoscale characterization of Escherichia coli biofilm formed under laminar flow using atomic force microscopy (AFM) and scanning electron microscopy (SEM). In: Bulletin of the Korean Chemical Society. 2008 ; Vol. 29, No. 11. pp. 2114-2118.
@article{a7aebca6ee894e85af9608f9b49d4302,
title = "Nanoscale characterization of Escherichia coli biofilm formed under laminar flow using atomic force microscopy (AFM) and scanning electron microscopy (SEM)",
abstract = "Biofilm contains heterogeneous three-dimensional structures composed of extracellular polymeric substance (EPS), which are greatly influenced by flow conditions. Here, we report a microfluidic platform highly suitable for nanoscale investigation of biofilms formed under laminar flows. This is possible because biofilms formed on glass beads having an average diameter of about 200 μm in the microfluidic device can be easily taken out and located for imaging under high resolution microscopes, such as atomic force microscope (AFM) and scanning electron microscope (SEM). Escherichia coli formed biofilms in the device at various flow conditions (0-50 μL min-1) for several days. SEM showed nanopores (14-100 nm) in biofilm at lower flow rates (0.5 and 5 μL min-1) only at within 3 days, while such small pores were not observed at higher flow rates (50 μL min-1) during the entire culture period (0-5 days). AFM results showed that the surface coverage and roughness of biofilm increased as the flow rate increased. These results suggest that like turbulent flow regime flow rates under laminar flow regime greatly influence on the morphology of biofilms in both micro- and nanoscales.",
keywords = "AFM, Biofilm, Escherichia coli O157:H7, Nanostructure, SEM",
author = "Jeesun Lim and Lee, {Kang Mu} and So, {Hyun Kim} and Nam, {Seong Won} and Yoo, {Jin Oh} and Hyun, {Sun Yun} and William Jo and Sejong Oh and Kim, {Sae Hun} and Sungsu Park",
year = "2008",
month = "11",
day = "20",
language = "English",
volume = "29",
pages = "2114--2118",
journal = "Bulletin of the Korean Chemical Society",
issn = "0253-2964",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Nanoscale characterization of Escherichia coli biofilm formed under laminar flow using atomic force microscopy (AFM) and scanning electron microscopy (SEM)

AU - Lim, Jeesun

AU - Lee, Kang Mu

AU - So, Hyun Kim

AU - Nam, Seong Won

AU - Yoo, Jin Oh

AU - Hyun, Sun Yun

AU - Jo, William

AU - Oh, Sejong

AU - Kim, Sae Hun

AU - Park, Sungsu

PY - 2008/11/20

Y1 - 2008/11/20

N2 - Biofilm contains heterogeneous three-dimensional structures composed of extracellular polymeric substance (EPS), which are greatly influenced by flow conditions. Here, we report a microfluidic platform highly suitable for nanoscale investigation of biofilms formed under laminar flows. This is possible because biofilms formed on glass beads having an average diameter of about 200 μm in the microfluidic device can be easily taken out and located for imaging under high resolution microscopes, such as atomic force microscope (AFM) and scanning electron microscope (SEM). Escherichia coli formed biofilms in the device at various flow conditions (0-50 μL min-1) for several days. SEM showed nanopores (14-100 nm) in biofilm at lower flow rates (0.5 and 5 μL min-1) only at within 3 days, while such small pores were not observed at higher flow rates (50 μL min-1) during the entire culture period (0-5 days). AFM results showed that the surface coverage and roughness of biofilm increased as the flow rate increased. These results suggest that like turbulent flow regime flow rates under laminar flow regime greatly influence on the morphology of biofilms in both micro- and nanoscales.

AB - Biofilm contains heterogeneous three-dimensional structures composed of extracellular polymeric substance (EPS), which are greatly influenced by flow conditions. Here, we report a microfluidic platform highly suitable for nanoscale investigation of biofilms formed under laminar flows. This is possible because biofilms formed on glass beads having an average diameter of about 200 μm in the microfluidic device can be easily taken out and located for imaging under high resolution microscopes, such as atomic force microscope (AFM) and scanning electron microscope (SEM). Escherichia coli formed biofilms in the device at various flow conditions (0-50 μL min-1) for several days. SEM showed nanopores (14-100 nm) in biofilm at lower flow rates (0.5 and 5 μL min-1) only at within 3 days, while such small pores were not observed at higher flow rates (50 μL min-1) during the entire culture period (0-5 days). AFM results showed that the surface coverage and roughness of biofilm increased as the flow rate increased. These results suggest that like turbulent flow regime flow rates under laminar flow regime greatly influence on the morphology of biofilms in both micro- and nanoscales.

KW - AFM

KW - Biofilm

KW - Escherichia coli O157:H7

KW - Nanostructure

KW - SEM

UR - http://www.scopus.com/inward/record.url?scp=57349127481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57349127481&partnerID=8YFLogxK

M3 - Article

VL - 29

SP - 2114

EP - 2118

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

SN - 0253-2964

IS - 11

ER -