Na+/Ca2+ exchanger 2 is neuroprotective by exporting Ca2+ during a transient focal cerebral ischemia in the mouse

Daejong Jeon, Kon Chu, Keun Hwa Jung, Manho Kim, Byung Woo Yoon, Changjoon Lee, Uhtaek Oh, Hee Sup Shin

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Na+/Ca2+ exchanger (NCX), by mediating Na+ and Ca2+ fluxes bi-directionally, assumes a role in controlling the Ca2+ homeostasis in the ischemic brain. It has been suggested that the three isoforms of NCX (NCX1, 2 and 3) may be differentially involved in permanent cerebral ischemia. However, the role of NCX2 has not been defined in ischemic reperfusion injury after a transient focal cerebral ischemia. Furthermore, it is not known whether NCX2 imports or exports intracellular Ca2+ ([Ca2+]i) following ischemia and reperfusion. To define the role of NCX2 in ischemia and reperfusion, we examined mice lacking NCX2, in vivo and in vitro. After an in vitro ischemia, a significantly slower recovery in population spike amplitudes, a sustained elevation of [Ca2+]i and an increased membrane depolarization were developed in the NCX2-deficient hippocampus. Moreover, a transient focal cerebral ischemia in vivo produced a larger infarction and more cell death in the NCX2-deficient mouse brain. In particular, in the wild type brain, NCX2-expressing neurons were largely spared from cell death after ischemia. Our results suggest that NCX2 exports Ca2+ in ischemia and thus protects neuronal cells from death by reducing [Ca2+]i in the adult mouse brain.

Original languageEnglish
Pages (from-to)482-491
Number of pages10
JournalCell Calcium
Volume43
Issue number5
DOIs
Publication statusPublished - 2008 May 1
Externally publishedYes

Fingerprint

Transient Ischemic Attack
Ischemia
Cell Death
Brain
Reperfusion
Reperfusion Injury
Brain Ischemia
Infarction
Hippocampus
Protein Isoforms
Homeostasis
Neurons
Membranes
Population
In Vitro Techniques

Keywords

  • [Ca]
  • Cerebral ischemia
  • Membrane depolarization
  • Na/Ca exchanger
  • NCX2-deficient mice
  • Population spike

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

Cite this

Na+/Ca2+ exchanger 2 is neuroprotective by exporting Ca2+ during a transient focal cerebral ischemia in the mouse. / Jeon, Daejong; Chu, Kon; Jung, Keun Hwa; Kim, Manho; Yoon, Byung Woo; Lee, Changjoon; Oh, Uhtaek; Shin, Hee Sup.

In: Cell Calcium, Vol. 43, No. 5, 01.05.2008, p. 482-491.

Research output: Contribution to journalArticle

Jeon, Daejong ; Chu, Kon ; Jung, Keun Hwa ; Kim, Manho ; Yoon, Byung Woo ; Lee, Changjoon ; Oh, Uhtaek ; Shin, Hee Sup. / Na+/Ca2+ exchanger 2 is neuroprotective by exporting Ca2+ during a transient focal cerebral ischemia in the mouse. In: Cell Calcium. 2008 ; Vol. 43, No. 5. pp. 482-491.
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