Near-infrared plasmonic assemblies of gold nanoparticles with multimodal function for targeted cancer theragnosis

Seong Eun Kim, Bo Ram Lee, Hohyeon Lee, Sung Duk Jo, Hyuncheol Kim, You Yeon Won, Jeewon Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Here we report a novel assembly structure of near-infrared plasmonic gold nanoparticles (AuNPs), possessing both photoacoustic (PA) and photothermal (PT) properties. The template for the plasmonic AuNP assembly is a bioconjugate between short double-strand DNA (sh-dsDNA) and human methyl binding domain protein 1 (MBD1). MBD1 binds to methylated cytosine-guanine dinucleotides (mCGs) within the sequence of sh-dsDNA. Hexahistidine peptides on the engineered MBD1 function as a nucleation site for AuNP synthesis, allowing the construction of hybrid conjugates, sh-dsDNA-MBD1-AuNPs (named DMAs). By varying the length of sh-dsDNA backbone and the spacer between two adjacent mCGs, we synthesized three different DMAs (DMA_5mCG, DMA_9mCG, and DMA_21mCG), among which DMA_21mCG exhibited a comparable photothermal and surprisingly a higher photoacoustic signals, compared to a plasmonic gold nanorod. Further, epidermal growth factor receptor I (EGFR)-binding peptides are genetically attached to the MBD1 of DMA_21mCG, enabling its efficient endocytosis into EGFR-overexpressing cancer cells. Notably, the denaturation of MBD1 disassembled the DMA and accordingly released the individual small AuNPs (<5 nm) that can be easily cleared from the body through renal excretion without causing accumulation/toxicity problems. This DMA-based novel approach offers a promising platform for targeted cancer theragnosis based on simultaneous PA imaging and PT therapy.

Original languageEnglish
Article number17327
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Near-infrared plasmonic assemblies of gold nanoparticles with multimodal function for targeted cancer theragnosis'. Together they form a unique fingerprint.

  • Cite this