Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity

Moon Hee Lee, Jong Hwa Jang, Gun Young Yoon, Seung Jun Lee, Min Goo Lee, Tae Heung Kang, Hee Dong Han, Hyuk Soon Kim, Wahn Soo Choi, Won Sun Park, Yeong Min Park, In Duk Jung

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.

Original languageEnglish
Pages (from-to)263-268
Number of pages6
JournalBMB reports
Volume50
Issue number5
DOIs
Publication statusPublished - 2017

Keywords

  • Antitumor immunity
  • Dendritic cells
  • Natural killer cells
  • Neoagarohexaose
  • Toll-like receptor 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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