Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity

Moon Hee Lee, Jong Hwa Jang, Gun Young Yoon, Seung Jun Lee, Min-Goo Lee, Tae Heung Kang, Hee Dong Han, Hyuk Soon Kim, Wahn Soo Choi, Won Sun Park, Yeong Min Park, In Duk Jung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.

Original languageEnglish
Pages (from-to)263-268
Number of pages6
JournalBMB Reports
Volume50
Issue number5
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Toll-Like Receptor 4
Natural Killer Cells
Dendritic Cells
Immunity
Chemical activation
Melanoma
Modulators
Agar
Antibodies
agarase

Keywords

  • Antitumor immunity
  • Dendritic cells
  • Natural killer cells
  • Neoagarohexaose
  • Toll-like receptor 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity. / Lee, Moon Hee; Jang, Jong Hwa; Yoon, Gun Young; Lee, Seung Jun; Lee, Min-Goo; Kang, Tae Heung; Han, Hee Dong; Kim, Hyuk Soon; Choi, Wahn Soo; Park, Won Sun; Park, Yeong Min; Jung, In Duk.

In: BMB Reports, Vol. 50, No. 5, 01.01.2017, p. 263-268.

Research output: Contribution to journalArticle

Lee, Moon Hee ; Jang, Jong Hwa ; Yoon, Gun Young ; Lee, Seung Jun ; Lee, Min-Goo ; Kang, Tae Heung ; Han, Hee Dong ; Kim, Hyuk Soon ; Choi, Wahn Soo ; Park, Won Sun ; Park, Yeong Min ; Jung, In Duk. / Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity. In: BMB Reports. 2017 ; Vol. 50, No. 5. pp. 263-268.
@article{ba8abde8cf994fc5a3aaac324367f7be,
title = "Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity",
abstract = "β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.",
keywords = "Antitumor immunity, Dendritic cells, Natural killer cells, Neoagarohexaose, Toll-like receptor 4",
author = "Lee, {Moon Hee} and Jang, {Jong Hwa} and Yoon, {Gun Young} and Lee, {Seung Jun} and Min-Goo Lee and Kang, {Tae Heung} and Han, {Hee Dong} and Kim, {Hyuk Soon} and Choi, {Wahn Soo} and Park, {Won Sun} and Park, {Yeong Min} and Jung, {In Duk}",
year = "2017",
month = "1",
day = "1",
doi = "10.5483/BMBRep.2017.50.5.014",
language = "English",
volume = "50",
pages = "263--268",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "5",

}

TY - JOUR

T1 - Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity

AU - Lee, Moon Hee

AU - Jang, Jong Hwa

AU - Yoon, Gun Young

AU - Lee, Seung Jun

AU - Lee, Min-Goo

AU - Kang, Tae Heung

AU - Han, Hee Dong

AU - Kim, Hyuk Soon

AU - Choi, Wahn Soo

AU - Park, Won Sun

AU - Park, Yeong Min

AU - Jung, In Duk

PY - 2017/1/1

Y1 - 2017/1/1

N2 - β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.

AB - β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.

KW - Antitumor immunity

KW - Dendritic cells

KW - Natural killer cells

KW - Neoagarohexaose

KW - Toll-like receptor 4

UR - http://www.scopus.com/inward/record.url?scp=85020030223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020030223&partnerID=8YFLogxK

U2 - 10.5483/BMBRep.2017.50.5.014

DO - 10.5483/BMBRep.2017.50.5.014

M3 - Article

C2 - 28287066

AN - SCOPUS:85020030223

VL - 50

SP - 263

EP - 268

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 5

ER -