Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity

Moon Hee Lee, Jong Hwa Jang, Gun Young Yoon, Seung Jun Lee, Min-Goo Lee, Tae Heung Kang, Hee Dong Han, Hyuk Soon Kim, Wahn Soo Choi, Won Sun Park, Yeong Min Park, In Duk Jung

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10 Citations (Scopus)

Abstract

β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.

Original languageEnglish
Pages (from-to)263-268
Number of pages6
JournalBMB Reports
Volume50
Issue number5
DOIs
Publication statusPublished - 2017 Jan 1

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Keywords

  • Antitumor immunity
  • Dendritic cells
  • Natural killer cells
  • Neoagarohexaose
  • Toll-like receptor 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Lee, M. H., Jang, J. H., Yoon, G. Y., Lee, S. J., Lee, M-G., Kang, T. H., Han, H. D., Kim, H. S., Choi, W. S., Park, W. S., Park, Y. M., & Jung, I. D. (2017). Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity. BMB Reports, 50(5), 263-268. https://doi.org/10.5483/BMBRep.2017.50.5.014