Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Jimann Shin, Hae Chul Park, Jolanta M. Topczewska, David J. Madwsley, Bruce Appel

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalMethods in Cell Science
Volume25
Issue number1-2
DOIs
Publication statusPublished - 2003 Dec 1
Externally publishedYes

Fingerprint

Zebrafish
Gene expression
Image analysis
DNA
Clone Cells
Reporter Genes
Embryonic Structures
Gene Expression

Keywords

  • Cerebellum
  • EGFP
  • Motor neuron
  • Olig2
  • Oligodendrocyte
  • Purkinje neuron
  • Spinal cord
  • Transgenic
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology

Cite this

Neural cell fate analysis in zebrafish using olig2 BAC transgenics. / Shin, Jimann; Park, Hae Chul; Topczewska, Jolanta M.; Madwsley, David J.; Appel, Bruce.

In: Methods in Cell Science, Vol. 25, No. 1-2, 01.12.2003, p. 7-14.

Research output: Contribution to journalArticle

Shin, Jimann ; Park, Hae Chul ; Topczewska, Jolanta M. ; Madwsley, David J. ; Appel, Bruce. / Neural cell fate analysis in zebrafish using olig2 BAC transgenics. In: Methods in Cell Science. 2003 ; Vol. 25, No. 1-2. pp. 7-14.
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