Abstract
Generation of a monomethylated selenium metabolite is critical for the anticancer activity of selenium. Because of its strong nucleophilicity, the metabolite can react directly with protein thiols to cause redox modification. Here, we report a neural network-based analysis to identify potential selenium targets. A reactive thiol specific reagent, BIAM, was used to monitor thiol proteome changes on 2D gel. We constructed a dynamic model and evaluated the relative importance of proteins mediating the cellular responses to selenium. Information from this study will provide new clues to unravel mechanisms of anticancer action of selenium. High impact selenium targets could also serve as biomarkers to gauge the efficacy of selenium chemoprevention.
| Original language | English |
|---|---|
| Pages (from-to) | 37-64 |
| Number of pages | 28 |
| Journal | Preparative Biochemistry and Biotechnology |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Feb 1 |
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Keywords
- Anticancer action
- Display thiol proteomics
- Dynamic modeling
- Neural-network
- Redox modification
- Selenium
ASJC Scopus subject areas
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Biotechnology
Cite this
Neural network-based analysis of thiol proteomics data in identifying potential selenium targets. / Lee, Jong Sik; Ma, Yong Beom; Choi, Kyoung Soo; Park, Soo Yeon; Baek, Sun Hee; Park, Young Mee; Zu, Ke; Zhang, Haitao; Ip, Clement; Kim, Yeul Hong; Park, Eun Mi.
In: Preparative Biochemistry and Biotechnology, Vol. 36, No. 1, 01.02.2006, p. 37-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neural network-based analysis of thiol proteomics data in identifying potential selenium targets
AU - Lee, Jong Sik
AU - Ma, Yong Beom
AU - Choi, Kyoung Soo
AU - Park, Soo Yeon
AU - Baek, Sun Hee
AU - Park, Young Mee
AU - Zu, Ke
AU - Zhang, Haitao
AU - Ip, Clement
AU - Kim, Yeul Hong
AU - Park, Eun Mi
PY - 2006/2/1
Y1 - 2006/2/1
N2 - Generation of a monomethylated selenium metabolite is critical for the anticancer activity of selenium. Because of its strong nucleophilicity, the metabolite can react directly with protein thiols to cause redox modification. Here, we report a neural network-based analysis to identify potential selenium targets. A reactive thiol specific reagent, BIAM, was used to monitor thiol proteome changes on 2D gel. We constructed a dynamic model and evaluated the relative importance of proteins mediating the cellular responses to selenium. Information from this study will provide new clues to unravel mechanisms of anticancer action of selenium. High impact selenium targets could also serve as biomarkers to gauge the efficacy of selenium chemoprevention.
AB - Generation of a monomethylated selenium metabolite is critical for the anticancer activity of selenium. Because of its strong nucleophilicity, the metabolite can react directly with protein thiols to cause redox modification. Here, we report a neural network-based analysis to identify potential selenium targets. A reactive thiol specific reagent, BIAM, was used to monitor thiol proteome changes on 2D gel. We constructed a dynamic model and evaluated the relative importance of proteins mediating the cellular responses to selenium. Information from this study will provide new clues to unravel mechanisms of anticancer action of selenium. High impact selenium targets could also serve as biomarkers to gauge the efficacy of selenium chemoprevention.
KW - Anticancer action
KW - Display thiol proteomics
KW - Dynamic modeling
KW - Neural-network
KW - Redox modification
KW - Selenium
UR - http://www.scopus.com/inward/record.url?scp=31544431782&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31544431782&partnerID=8YFLogxK
U2 - 10.1080/10826060500388512
DO - 10.1080/10826060500388512
M3 - Article
VL - 36
SP - 37
EP - 64
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
SN - 1082-6068
IS - 1
ER -