Neural probes with multi-drug delivery capability

Hyogeun Shin, Hyunjoo J. Lee, Uikyu Chae, Huiyoung Kim, Jeongyeon Kim, Nakwon Choi, Jiwan Woo, Yakdol Cho, Changjoon Lee, Eui Sung Yoon, Il Joo Cho

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Multi-functional neural probes are promising platforms to conduct efficient and effective in-depth studies of brain by recording neural signals as well as modulating the signals with various stimuli. Here we present a neural probe with an embedded microfluidic channel (chemtrode) with multi-drug delivery capability suitable for small animal experiments. We integrated a staggered herringbone mixer (SHM) in a 3-inlet microfluidic chip directly into our chemtrode. This chip, which also serves as a compact interface for the chemtrode, allows for efficient delivery of small volumes of multiple or concentration-modulated drugs via chaotic mixing. We demonstrated the successful infusion of combinatorial inputs of three chemicals with a low flow rate (170 nl min<sup>-1</sup>). By sequentially delivering red, green, and blue inks from each inlet and conducting visual inspections at the tip of the chemtrode, we measured a short residence time of 14 s which corresponds to a small swept volume of 66 nl. Finally, we demonstrated the potential of our proposed chemtrode as an enabling tool through extensive in vivo mice experiments. Through simultaneous infusions of a chemical (pilocarpine or tetrodotoxin (TTX) at inlet 1), a buffer solution (saline at inlet 2), and 4′,6-diamidino-2-phenylindole (DAPI at inlet 3) locally into a mouse brain, we not only modulated the neural activities by varying the concentration of the chemical but also locally stained the cells at our target region (CA1 in hippocampus). More specifically, infusion of pilocarpine with a higher concentration resulted in an increase in neural activities while infusion of TTX with a higher concentration resulted in a distinctive reduction. For each chemical, we acquired multiple sets of data using only one mouse through a single implantation of the chemtrode. Our proposed chemtrode offers 1) multiplexed delivery of three drugs through a compact packaging with a small swept volume and 2) simultaneous recording to monitor near real-time effects on neural signals, which allows for more versatile in vivo experiments with a minimum number of animals to be sacrificed.

Original languageEnglish
Pages (from-to)3730-3737
Number of pages8
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume15
Issue number18
DOIs
Publication statusPublished - 2015 Jul 20
Externally publishedYes

Fingerprint

Drug delivery
Pilocarpine
Microfluidics
Tetrodotoxin
Pharmaceutical Preparations
Brain
Ink
Animals
Product Packaging
Sodium Chloride
Experiments
Hippocampus
Buffers
Packaging
Inspection
Flow rate

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Shin, H., Lee, H. J., Chae, U., Kim, H., Kim, J., Choi, N., ... Cho, I. J. (2015). Neural probes with multi-drug delivery capability. Lab on a Chip - Miniaturisation for Chemistry and Biology, 15(18), 3730-3737. https://doi.org/10.1039/c5lc00582e

Neural probes with multi-drug delivery capability. / Shin, Hyogeun; Lee, Hyunjoo J.; Chae, Uikyu; Kim, Huiyoung; Kim, Jeongyeon; Choi, Nakwon; Woo, Jiwan; Cho, Yakdol; Lee, Changjoon; Yoon, Eui Sung; Cho, Il Joo.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 15, No. 18, 20.07.2015, p. 3730-3737.

Research output: Contribution to journalArticle

Shin, H, Lee, HJ, Chae, U, Kim, H, Kim, J, Choi, N, Woo, J, Cho, Y, Lee, C, Yoon, ES & Cho, IJ 2015, 'Neural probes with multi-drug delivery capability', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 15, no. 18, pp. 3730-3737. https://doi.org/10.1039/c5lc00582e
Shin, Hyogeun ; Lee, Hyunjoo J. ; Chae, Uikyu ; Kim, Huiyoung ; Kim, Jeongyeon ; Choi, Nakwon ; Woo, Jiwan ; Cho, Yakdol ; Lee, Changjoon ; Yoon, Eui Sung ; Cho, Il Joo. / Neural probes with multi-drug delivery capability. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2015 ; Vol. 15, No. 18. pp. 3730-3737.
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