Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells

Byung Kak Kim, Sung Eun Kim, Joong Hyun Shim, Dong Hun Woo, Jung Eun Gil, Suel Kee Kim, Jong Hoon Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF), a potent mitogen for vascular endothelial cells, has been suggested as a modulator that is involved in neurogenesis as well as angiogenesis. Here, we directly examined the effect of VEGF on neuroectodermal differentiation using human embryonic stem cells (hESCs). VEGF treatment upregulated the expression of neuroectodermal genes (Sox1 and Nestin) during germ layer formation in embryoid bodies (EBs) and efficiently increased the number of neural rosettes expressing both Pax6 and Nestin. The neural progenitors generated from VEGF-treated EBs further differentiated into cells that showed a similar pattern of gene expression observed in the development of dopaminergic neurons upon terminal differentiation. These results support the neurogenic effect of VEGF on hESC differentiation.

Original languageEnglish
Pages (from-to)5869-5874
Number of pages6
JournalFEBS Letters
Volume580
Issue number25
DOIs
Publication statusPublished - 2006 Oct 30

Keywords

  • Differentiation
  • Embryonic germ layers
  • Human embryonic stem cells
  • Neurons
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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