Neuroglobin protects neurons against oxidative stress in global ischemia

Richard Changxun Li, Shang Zhi Guo, Seung Kwan Lee, David Gozal

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Neuroglobin (Ngb) is a recently discovered globin that affords protection against hypoxic/ischemic-induced cell injury in brain. Hypoxic/ischemic injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS). In previous studies, we found that Ngb has antioxidative properties, and protects PC-12 cells against hypoxia- and Β-amyloid-induced cell death. To further delineate the potential role of Ngb in protection against cerebral ischemia-reperfusion injury in vivo, we developed a transgenic mouse line that overexpresses Ngb. Hippocampal ischemia-reperfusion injury was induced by a 10-minute bilateral occlusion of the common carotid arteries, and the animal brains were assessed 3 days later. CA1 neural injury was determined by cresyl violet staining. Lipid peroxidation was assessed using a malonyldialdehyde assay kit, whereas ROS/RNS accumulation was determined by Het staining in the CA1 hippocampal region. Hippocampal Ngb mRNA and protein expressions were assessed by reverse transcriptase-PCR and western blotting, respectively. Neuroglobin was successfully overexpressed in the hippocampus of Ngb transgenic mice. After ischemia-reperfusion, CA1 ROS/RNS production and lipid peroxidation were markedly decreased in Ngb transgenic mice compared with wild-type mice. Furthermore, CA1 neuronal injury was also markedly reduced. Thus, Ngb may confer protection against ischemia-reperfusion injury in the brain through its intrinsic antioxidant properties.

Original languageEnglish
Pages (from-to)1874-1882
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume30
Issue number11
DOIs
Publication statusPublished - 2010 Nov 1

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Oxidative Stress
Ischemia
Neurons
Reactive Nitrogen Species
Reperfusion Injury
Transgenic Mice
Reactive Oxygen Species
Lipid Peroxidation
Wounds and Injuries
Staining and Labeling
Hippocampal CA1 Region
neuroglobin
Cell Hypoxia
Globins
Common Carotid Artery
Brain
Brain Ischemia
Malondialdehyde
Reverse Transcriptase Polymerase Chain Reaction
Amyloid

Keywords

  • global ischemia
  • hippocampus
  • neuroglobin
  • neuron
  • oxidative stress
  • transgenic mouse

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Neuroglobin protects neurons against oxidative stress in global ischemia. / Li, Richard Changxun; Guo, Shang Zhi; Lee, Seung Kwan; Gozal, David.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 30, No. 11, 01.11.2010, p. 1874-1882.

Research output: Contribution to journalArticle

Li, Richard Changxun ; Guo, Shang Zhi ; Lee, Seung Kwan ; Gozal, David. / Neuroglobin protects neurons against oxidative stress in global ischemia. In: Journal of Cerebral Blood Flow and Metabolism. 2010 ; Vol. 30, No. 11. pp. 1874-1882.
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