Abstract
Excessive accumulation of amyloid beta (Aβ) has been proposed as a pivotal event in the pathogenesis of Alzheimer's disease. Possible mechanisms underlying Aβ-induced neuronal cytotoxicity include excess production of reactive oxidative species (ROS) and apoptosis. Neuroglobin (Ngb), a newly discovered globin in vertebrates that exhibits neuroprotective functions, may have a potential role in scavenging ROS. To examine the potential protective role of Ngb in Aβ-induced cytotoxicity, PC12 cells were treated with Aβ (1-42 fragment) for 24 h. Aβ treatments increased ROS production in PC12 cells. Overexpression of Ngb but not Ngb mutant in the PC12 cells significantly attenuated Aβ-induced ROS production and lipids peroxidation. Furthermore, overexpression of Ngb also attenuated Aβ-induced mitochondrial dysfunction and apoptosis, and promoted cell survival in PC12 cells. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury.
| Original language | English |
|---|---|
| Pages (from-to) | 1815-1822 |
| Number of pages | 8 |
| Journal | Neurobiology of Aging |
| Volume | 29 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2008 Dec 1 |
| Externally published | Yes |
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Keywords
- Amyloid
- Apoptosis
- Neuroglobin
- Oxidative stress
ASJC Scopus subject areas
- Neuroscience(all)
- Ageing
- Clinical Neurology
- Developmental Biology
- Geriatrics and Gerontology
Cite this
Neuroglobin protects PC12 cells against β-amyloid-induced cell injury. / Li, Richard C.; Pouranfar, Farzan; Lee, Seung Kwan; Morris, Matthew W.; Wang, Yang; Gozal, David.
In: Neurobiology of Aging, Vol. 29, No. 12, 01.12.2008, p. 1815-1822.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuroglobin protects PC12 cells against β-amyloid-induced cell injury
AU - Li, Richard C.
AU - Pouranfar, Farzan
AU - Lee, Seung Kwan
AU - Morris, Matthew W.
AU - Wang, Yang
AU - Gozal, David
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Excessive accumulation of amyloid beta (Aβ) has been proposed as a pivotal event in the pathogenesis of Alzheimer's disease. Possible mechanisms underlying Aβ-induced neuronal cytotoxicity include excess production of reactive oxidative species (ROS) and apoptosis. Neuroglobin (Ngb), a newly discovered globin in vertebrates that exhibits neuroprotective functions, may have a potential role in scavenging ROS. To examine the potential protective role of Ngb in Aβ-induced cytotoxicity, PC12 cells were treated with Aβ (1-42 fragment) for 24 h. Aβ treatments increased ROS production in PC12 cells. Overexpression of Ngb but not Ngb mutant in the PC12 cells significantly attenuated Aβ-induced ROS production and lipids peroxidation. Furthermore, overexpression of Ngb also attenuated Aβ-induced mitochondrial dysfunction and apoptosis, and promoted cell survival in PC12 cells. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury.
AB - Excessive accumulation of amyloid beta (Aβ) has been proposed as a pivotal event in the pathogenesis of Alzheimer's disease. Possible mechanisms underlying Aβ-induced neuronal cytotoxicity include excess production of reactive oxidative species (ROS) and apoptosis. Neuroglobin (Ngb), a newly discovered globin in vertebrates that exhibits neuroprotective functions, may have a potential role in scavenging ROS. To examine the potential protective role of Ngb in Aβ-induced cytotoxicity, PC12 cells were treated with Aβ (1-42 fragment) for 24 h. Aβ treatments increased ROS production in PC12 cells. Overexpression of Ngb but not Ngb mutant in the PC12 cells significantly attenuated Aβ-induced ROS production and lipids peroxidation. Furthermore, overexpression of Ngb also attenuated Aβ-induced mitochondrial dysfunction and apoptosis, and promoted cell survival in PC12 cells. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury.
KW - Amyloid
KW - Apoptosis
KW - Neuroglobin
KW - Oxidative stress
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U2 - 10.1016/j.neurobiolaging.2007.05.001
DO - 10.1016/j.neurobiolaging.2007.05.001
M3 - Article
C2 - 17560688
AN - SCOPUS:53649088015
VL - 29
SP - 1815
EP - 1822
JO - Neurobiology of Aging
JF - Neurobiology of Aging
SN - 0197-4580
IS - 12
ER -