Neuroglobin protects PC12 cells against β-amyloid-induced cell injury

Richard C. Li, Farzan Pouranfar, Seung Kwan Lee, Matthew W. Morris, Yang Wang, David Gozal

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Excessive accumulation of amyloid beta (Aβ) has been proposed as a pivotal event in the pathogenesis of Alzheimer's disease. Possible mechanisms underlying Aβ-induced neuronal cytotoxicity include excess production of reactive oxidative species (ROS) and apoptosis. Neuroglobin (Ngb), a newly discovered globin in vertebrates that exhibits neuroprotective functions, may have a potential role in scavenging ROS. To examine the potential protective role of Ngb in Aβ-induced cytotoxicity, PC12 cells were treated with Aβ (1-42 fragment) for 24 h. Aβ treatments increased ROS production in PC12 cells. Overexpression of Ngb but not Ngb mutant in the PC12 cells significantly attenuated Aβ-induced ROS production and lipids peroxidation. Furthermore, overexpression of Ngb also attenuated Aβ-induced mitochondrial dysfunction and apoptosis, and promoted cell survival in PC12 cells. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury.

Original languageEnglish
Pages (from-to)1815-1822
Number of pages8
JournalNeurobiology of Aging
Volume29
Issue number12
DOIs
Publication statusPublished - 2008 Dec

Keywords

  • Amyloid
  • Apoptosis
  • Neuroglobin
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)
  • Ageing
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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