Abstract
Neuroglobin (Ngb) is a newly discovered globin in the vertebrate brain that exhibits neuroprotection against hypoxic/ischemic injury. Hypoxic/ischemic brain injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS), and antioxidants or ROS scavengers promote cell survival. Therefore, Ngb may serve as a scavenger of toxic reactive species, such as hydrogen peroxide. To examine the anti-oxidative role of neuroglobin, PC12 cells were transfected with wild type and mutant (H64 V/H96A) Ngb for 48 h and then treated with H 2 O 2 (0.1, 0.2 and 0.4 mM) for 6 h. Ngb siRNA decreased the H 2 O 2 -induced Ngb expression and exacerbated H 2 O 2 -induced cell injury. Transient transfection of Ngb induced dose-dependent increases in Ngb protein expression and did not alter SOD, GPX, and catalase activities. Overexpression of wild type Ngb, but not of mutant Ngb, significantly attenuated H 2 O 2 -induced ROS/RNS accumulation and lipid peroxidation, decreased H 2 O 2 -induced mitochondrial dysfunction and apoptosis, and promoted overall cell survival. Thus, Ngb plays a protective role against oxidative stress, which appears to be primarily mediated by intrinsic Ngb antioxidant properties.
| Original language | English |
|---|---|
| Pages (from-to) | 159-166 |
| Number of pages | 8 |
| Journal | Brain Research |
| Volume | 1190 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Jan 23 |
| Externally published | Yes |
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Keywords
- Neuroglobin
- Oxidative stress
- PC12 cell
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Cite this
Neuroglobin protects PC12 cells against oxidative stress. / Li, Richard C.; Morris, Matthew W.; Lee, Seung Kwan; Pouranfar, Farzan; Wang, Yang; Gozal, David.
In: Brain Research, Vol. 1190, No. 1, 23.01.2008, p. 159-166.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuroglobin protects PC12 cells against oxidative stress
AU - Li, Richard C.
AU - Morris, Matthew W.
AU - Lee, Seung Kwan
AU - Pouranfar, Farzan
AU - Wang, Yang
AU - Gozal, David
PY - 2008/1/23
Y1 - 2008/1/23
N2 - Neuroglobin (Ngb) is a newly discovered globin in the vertebrate brain that exhibits neuroprotection against hypoxic/ischemic injury. Hypoxic/ischemic brain injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS), and antioxidants or ROS scavengers promote cell survival. Therefore, Ngb may serve as a scavenger of toxic reactive species, such as hydrogen peroxide. To examine the anti-oxidative role of neuroglobin, PC12 cells were transfected with wild type and mutant (H64 V/H96A) Ngb for 48 h and then treated with H 2 O 2 (0.1, 0.2 and 0.4 mM) for 6 h. Ngb siRNA decreased the H 2 O 2 -induced Ngb expression and exacerbated H 2 O 2 -induced cell injury. Transient transfection of Ngb induced dose-dependent increases in Ngb protein expression and did not alter SOD, GPX, and catalase activities. Overexpression of wild type Ngb, but not of mutant Ngb, significantly attenuated H 2 O 2 -induced ROS/RNS accumulation and lipid peroxidation, decreased H 2 O 2 -induced mitochondrial dysfunction and apoptosis, and promoted overall cell survival. Thus, Ngb plays a protective role against oxidative stress, which appears to be primarily mediated by intrinsic Ngb antioxidant properties.
AB - Neuroglobin (Ngb) is a newly discovered globin in the vertebrate brain that exhibits neuroprotection against hypoxic/ischemic injury. Hypoxic/ischemic brain injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS), and antioxidants or ROS scavengers promote cell survival. Therefore, Ngb may serve as a scavenger of toxic reactive species, such as hydrogen peroxide. To examine the anti-oxidative role of neuroglobin, PC12 cells were transfected with wild type and mutant (H64 V/H96A) Ngb for 48 h and then treated with H 2 O 2 (0.1, 0.2 and 0.4 mM) for 6 h. Ngb siRNA decreased the H 2 O 2 -induced Ngb expression and exacerbated H 2 O 2 -induced cell injury. Transient transfection of Ngb induced dose-dependent increases in Ngb protein expression and did not alter SOD, GPX, and catalase activities. Overexpression of wild type Ngb, but not of mutant Ngb, significantly attenuated H 2 O 2 -induced ROS/RNS accumulation and lipid peroxidation, decreased H 2 O 2 -induced mitochondrial dysfunction and apoptosis, and promoted overall cell survival. Thus, Ngb plays a protective role against oxidative stress, which appears to be primarily mediated by intrinsic Ngb antioxidant properties.
KW - Neuroglobin
KW - Oxidative stress
KW - PC12 cell
UR - http://www.scopus.com/inward/record.url?scp=37549003333&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37549003333&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2007.11.022
DO - 10.1016/j.brainres.2007.11.022
M3 - Article
VL - 1190
SP - 159
EP - 166
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -