Neuroglobin protects PC12 cells against oxidative stress

Richard C. Li, Matthew W. Morris, Seung Kwan Lee, Farzan Pouranfar, Yang Wang, David Gozal

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Neuroglobin (Ngb) is a newly discovered globin in the vertebrate brain that exhibits neuroprotection against hypoxic/ischemic injury. Hypoxic/ischemic brain injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS), and antioxidants or ROS scavengers promote cell survival. Therefore, Ngb may serve as a scavenger of toxic reactive species, such as hydrogen peroxide. To examine the anti-oxidative role of neuroglobin, PC12 cells were transfected with wild type and mutant (H64 V/H96A) Ngb for 48 h and then treated with H 2 O 2 (0.1, 0.2 and 0.4 mM) for 6 h. Ngb siRNA decreased the H 2 O 2 -induced Ngb expression and exacerbated H 2 O 2 -induced cell injury. Transient transfection of Ngb induced dose-dependent increases in Ngb protein expression and did not alter SOD, GPX, and catalase activities. Overexpression of wild type Ngb, but not of mutant Ngb, significantly attenuated H 2 O 2 -induced ROS/RNS accumulation and lipid peroxidation, decreased H 2 O 2 -induced mitochondrial dysfunction and apoptosis, and promoted overall cell survival. Thus, Ngb plays a protective role against oxidative stress, which appears to be primarily mediated by intrinsic Ngb antioxidant properties.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalBrain Research
Volume1190
Issue number1
DOIs
Publication statusPublished - 2008 Jan 23
Externally publishedYes

Fingerprint

PC12 Cells
Oxidative Stress
Reactive Nitrogen Species
Reactive Oxygen Species
neuroglobin
Cell Survival
Antioxidants
Globins
Poisons
Wounds and Injuries
Catalase
Brain Injuries
Hydrogen Peroxide
Small Interfering RNA
Lipid Peroxidation
Transfection
Vertebrates
Apoptosis

Keywords

  • Neuroglobin
  • Oxidative stress
  • PC12 cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Li, R. C., Morris, M. W., Lee, S. K., Pouranfar, F., Wang, Y., & Gozal, D. (2008). Neuroglobin protects PC12 cells against oxidative stress. Brain Research, 1190(1), 159-166. https://doi.org/10.1016/j.brainres.2007.11.022

Neuroglobin protects PC12 cells against oxidative stress. / Li, Richard C.; Morris, Matthew W.; Lee, Seung Kwan; Pouranfar, Farzan; Wang, Yang; Gozal, David.

In: Brain Research, Vol. 1190, No. 1, 23.01.2008, p. 159-166.

Research output: Contribution to journalArticle

Li, RC, Morris, MW, Lee, SK, Pouranfar, F, Wang, Y & Gozal, D 2008, 'Neuroglobin protects PC12 cells against oxidative stress', Brain Research, vol. 1190, no. 1, pp. 159-166. https://doi.org/10.1016/j.brainres.2007.11.022
Li, Richard C. ; Morris, Matthew W. ; Lee, Seung Kwan ; Pouranfar, Farzan ; Wang, Yang ; Gozal, David. / Neuroglobin protects PC12 cells against oxidative stress. In: Brain Research. 2008 ; Vol. 1190, No. 1. pp. 159-166.
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