Abstract
Vacuolar ATPase (V-ATPase) is a multi-subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V0 domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron-specific expression of atp6v0c2, a novel isoform of the V-ATPase c-subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome-wide analysis of the zebrafish mibta52b mutant designed to identify genes differentially regulated by Notch signaling. Whole-mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post-mitotic neurons. The ATP6V0C2 protein is co-localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss-of-function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability.
| Original language | English |
|---|---|
| Pages (from-to) | 2501-2508 |
| Number of pages | 8 |
| Journal | Developmental Dynamics |
| Volume | 239 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2010 Sep 1 |
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Keywords
- Neuron
- Notch signaling
- V-ATPase
- Zebrafish
ASJC Scopus subject areas
- Developmental Biology
Cite this
Neuron-specific expression of atp6v0c2 in zebrafish CNS. / Chung, Ah Young; Kim, Myoung Jin; Kim, Dohyun; Bang, Sangsu; Hwang, Sun Wook; Lim, Chae Seung; Lee, Sanggyu; Park, Hae Chul; Huh, Tae Lin.
In: Developmental Dynamics, Vol. 239, No. 9, 01.09.2010, p. 2501-2508.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuron-specific expression of atp6v0c2 in zebrafish CNS
AU - Chung, Ah Young
AU - Kim, Myoung Jin
AU - Kim, Dohyun
AU - Bang, Sangsu
AU - Hwang, Sun Wook
AU - Lim, Chae Seung
AU - Lee, Sanggyu
AU - Park, Hae Chul
AU - Huh, Tae Lin
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Vacuolar ATPase (V-ATPase) is a multi-subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V0 domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron-specific expression of atp6v0c2, a novel isoform of the V-ATPase c-subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome-wide analysis of the zebrafish mibta52b mutant designed to identify genes differentially regulated by Notch signaling. Whole-mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post-mitotic neurons. The ATP6V0C2 protein is co-localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss-of-function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability.
AB - Vacuolar ATPase (V-ATPase) is a multi-subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V0 domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron-specific expression of atp6v0c2, a novel isoform of the V-ATPase c-subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome-wide analysis of the zebrafish mibta52b mutant designed to identify genes differentially regulated by Notch signaling. Whole-mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post-mitotic neurons. The ATP6V0C2 protein is co-localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss-of-function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability.
KW - Neuron
KW - Notch signaling
KW - V-ATPase
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=77956294443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956294443&partnerID=8YFLogxK
U2 - 10.1002/dvdy.22383
DO - 10.1002/dvdy.22383
M3 - Article
C2 - 20839327
AN - SCOPUS:77956294443
VL - 239
SP - 2501
EP - 2508
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 9
ER -