Neuroprotective effect of human placental extract on hypoxic-ischemic brain injury in neonatal rats

Jee Yoon Park, Jung Hye Byeon, Sung Won Park, So Hee Eun, Kyu Young Chae, Baik Lin Eun

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    6 Citations (Scopus)

    Abstract

    We investigated the neuroprotective effects of human placental extracts (HPE) and the effects of HPE on recovery of cognitive and behavioral function on hypoxic-ischemic brain injury in the newborn rat. The right common carotid arteries of 7-day-old rats were coagulated, and rats were then exposed to 8% oxygen. Immediately before and again at three times after the hypoxia-ischemia (pre-treatment group), and immediately after and three times again after hypoxia-ischemia (post-treatment group), the rats were intraperitoneally injected with HPE (0.1, 0.25, or 0.5. mL/10. g/dose). No-treatment rats received saline only. On postnatal day 12, brains were removed and gross morphological damage was evaluated. To quantify the severity of brain injury, bilateral cross-sectional areas of the anterior commissural and posterior hippocampal levels were analyzed with NIH Image. Assessments of the open field activity levels at 2, 4, 6 and 8. week and, the Morris water maze test at 8. weeks after hypoxia-ischemia were carried out according to standard methods. HPE pre-treatment decreased the incidence of liquefactive cerebral infarction, at an optimally neuroprotective dose of 0.5. mL/10. g/dose (p<0.05). In the Morris water maze test, the group injected with HPE at 0.5. mL/10. g/dose concentration showed shorter escape latencies than the no-treatment group (p<0.05). These findings support a protective effect of the HPE treatment on neuronal integrity and cognitive function following hypoxic-ischemic brain injury. Injected at an appropriate dose prior to exposure, HPE may significantly reduce or prevent hypoxic-ischemic injury in the immature brain.

    Original languageEnglish
    Pages (from-to)68-74
    Number of pages7
    JournalBrain and Development
    Volume35
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jan

    Keywords

    • Cerebral ischemia
    • Human placental extract
    • Neuroprotection
    • Newborn

    ASJC Scopus subject areas

    • Pediatrics, Perinatology, and Child Health
    • Developmental Neuroscience
    • Clinical Neurology

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