Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals

Jieun Park, Jiyun Lee, Zia Yeom, Donghyuk Heo, Young Hee Lim

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Abstract

Recent evidence shows that the gut microbiota has an important role in gut-brain crosstalk and is linked to neuronal disorders. The aim of this study was to investigate the effects of intestinal Ruminococcus albus with probiotic potential on neuroprotection in oxidatively stressed SH-SY5Y neuroblastoma cells and animals. To investigate these effects, conditioned medium was prepared using Caco-2 cells cultured with heat-killed R. albus (CRA-CM). Caco-2 cells cultured with heat-killed R. albus showed increased BDNF expression and BDNF protein levels increased in CRA-CM. CRA-CM up-regulated the protein expression levels of SRF, C-fos and CDK2. In addition, CRA-CM protected SH-SY5Y cells from H2O2-induced cell death. CRA-CM significantly decreased the Bax/Bcl-2 ratio in oxidatively stressed SH-SY5Y cells. Animal experiments showed that oral administration of heat-killed R. albus for 15 days attenuated the oxidative stress induced by sodium arsenate. Treatment with heat-killed R. albus reduced the level of ROS, and the levels of SOD and GSH increased in oxidatively stressed brains. In conclusion, the secretome prepared from Caco-2 cells cultured with heat-killed R. albus might promote neuronal proliferation through the activation of cell proliferation-related proteins, and heat-killed R. albus protects neurons from oxidative damage by reducing ROS levels and increasing SOD and GSH levels.

Original languageEnglish
Article number14520
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Ruminococcus
Neuroprotective Agents
Hot Temperature
Caco-2 Cells
Brain-Derived Neurotrophic Factor
Proteins
Probiotics
Brain
Conditioned Culture Medium
Neuroblastoma
Oral Administration
Oxidative Stress
Cell Death
Cell Proliferation
Neurons

ASJC Scopus subject areas

  • General

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Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals. / Park, Jieun; Lee, Jiyun; Yeom, Zia; Heo, Donghyuk; Lim, Young Hee.

In: Scientific Reports, Vol. 7, No. 1, 14520, 01.12.2017.

Research output: Contribution to journalArticle

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