Wogonin (5,7-dihydroxy-8-methoxyflavone), an active component originated from the root of Scutellaria baicalensis Georgi, has been reported to possess antioxidant and anti-inflammatory properties. In this study, we investigated the neuroprotective effect of wogonin in a focal cerebral ischemia rat model. Wogonin markedly reduced the infarct volume after 2 h middle cerebral artery occlusion followed by 22 h reperfusion. Wogonin decreased the production of nitric oxide and inflammatory cytokines such as TNF-α and IL-6 in lipopolisaccharide-stimulated microglial cells. While wogonin reduced the activity of NF-κB, it did not change the activity of mitogen-activated protein kinases family members, p38, ERK and JNK. The lipopolisaccharide- stimulated production of NO and cytokines was significantly blocked by various kinds of NF-κB inhibitors such as N-acetyl cysteine, pyrrolidinedithiocarbamate and MG-132. The data may indicate that wogonin has neuroprotective effect by preventing the overactivation of microglial cells, possibly by inactivating NF-κB signaling pathway.
|Number of pages||7|
|Journal||Archives of pharmacal research|
|Publication status||Published - 2004 Sep 30|
ASJC Scopus subject areas
- Molecular Medicine
- Drug Discovery
- Organic Chemistry